Melatonin disrupts circadian rhythms of glutamate and GABA in the neostriatum of the awake rat: A microdialysis study

B. De Marquez Prado, T. R. Castañeda, A. Galindo, A. Del Arco, G. Segovia, R. J. Reiter, F. Mora

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


The purpose of this study was to investigate possible circadian changes in extracellular concentrations of glutamate (GLU) and γ-aminobutyric acid (GABA), and the influence of melatonin on the levels of these neurotransmitters in the neostriatum of awake rats using in vivo microdialysis. At the same time, the concentrations of the amino acids taurine (TAU), glutamine (GLN) and arginine (ARG), as well as dopamine (DA) and its metabolites 3, 4-dihydroxyphenyl acetic acid (DOPAC) and homovanillic acid (HVA), were measured in the extracellular fluid. When dialysates were collected over a 24-hr period (6 hr dark, 12 hr light, 6 hr dark), both GLU and GABA, without the infusion of melatonin, exhibited statistically significant rhythms, with higher levels of these constituents during the dark and lower levels during the day. Perfusion with melatonin (for 19 consecutive hours) prevented the daytime reductions in both GLU and GABA. Of the amino acids measured in the dialysates collected from the neostriatum of non-perfused rats, only ARG exhibited a significant change during the light:dark cycle; again, lowest concentrations were measured during the day. While melatonin perfusion did not statistically significantly influence neostriatal levels of TAU and ARG, GLN levels continued to drop during the infusion of the indoleamine. Dialysate concentrations of DA, DOPAC and HVA exhibited circadian rhythms which were not influenced by melatonin perfusion. The findings indicate there are differential effects of melatonin on extracellular neurotransmitter concentrations in the neostriatum of the awake rat. The results also suggest that the day:night variations in GLU and GABA may relate to daily changes in endogenous melatonin production, while DA and its metabolites are minimally influenced by this secretory product.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalJournal of pineal research
Issue number4
StatePublished - 2000
Externally publishedYes


  • Amino acid neurotransmitters
  • Dopamine and metabolites
  • Glutamate
  • Melatonin
  • Neostriatum
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Endocrinology


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