Abstract
Homocysteinemia is a major and independent risk factor for vascular disease. Oxidative stress is a possible mechanism for homocysteine (HCY)-induced vascular disease. Herein, we evaluated the antioxidant property of melatonin (MLT) in relation to the vasoconstrictive effect of HCY on the human umbilical artery. Helical umbilical arterial strips without endothelium were obtained at elective Cesarean delivery near term. Changes in potassium chloride (KCl)-induced vasoconstriction were measured. Arterial strips were treated with HCY (10 or 100 μM) plus FeSO4 (10 μM) alone or pretreated with a hydroxyl radical (·OH) scavenger, mannitol (20 mM), or MLT (1 or 10 μM). The effect of HCY on the response of arterial strips to external calcium (Ca2+) in the presence of KCl (20 mM) was determined. HCY plus FeSO4 potentiated KCl-induced vasoconstriction in a concentration-dependent manner; pretreatment with mannitol significantly reduced this vasospastic effect. HCY (100 μM) significantly augmented the contractile response to external Ca2+. MLT (10 μM) significantly suppressed the contractile response to external Ca2+. These results suggest that HCY potentiates KCl-induced umbilical artery vasoconstriction, in part by increasing Ca2+ influx in vascular smooth muscle cells via activation of Ca2+ channels. MLT significantly suppressed the vasoconstrictive effect of HCY, probably by scavenging ·OH arising from HCY autooxidation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 940-944 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 280 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Calcium
- Homocysteine
- Melatonin
- Umbilical artery
- Vasospasm
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Melatonin counteracts potentiation by homocysteine of KCl-induced vasoconstriction in human umbilical artery : Relation to calcium influx. / Okatani, Y.; Wakatsuki, A.; Reiter, Russel J.
In: Biochemical and Biophysical Research Communications, Vol. 280, No. 3, 2001, p. 940-944.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Melatonin counteracts potentiation by homocysteine of KCl-induced vasoconstriction in human umbilical artery
T2 - Relation to calcium influx
AU - Okatani, Y.
AU - Wakatsuki, A.
AU - Reiter, Russel J
PY - 2001
Y1 - 2001
N2 - Homocysteinemia is a major and independent risk factor for vascular disease. Oxidative stress is a possible mechanism for homocysteine (HCY)-induced vascular disease. Herein, we evaluated the antioxidant property of melatonin (MLT) in relation to the vasoconstrictive effect of HCY on the human umbilical artery. Helical umbilical arterial strips without endothelium were obtained at elective Cesarean delivery near term. Changes in potassium chloride (KCl)-induced vasoconstriction were measured. Arterial strips were treated with HCY (10 or 100 μM) plus FeSO4 (10 μM) alone or pretreated with a hydroxyl radical (·OH) scavenger, mannitol (20 mM), or MLT (1 or 10 μM). The effect of HCY on the response of arterial strips to external calcium (Ca2+) in the presence of KCl (20 mM) was determined. HCY plus FeSO4 potentiated KCl-induced vasoconstriction in a concentration-dependent manner; pretreatment with mannitol significantly reduced this vasospastic effect. HCY (100 μM) significantly augmented the contractile response to external Ca2+. MLT (10 μM) significantly suppressed the contractile response to external Ca2+. These results suggest that HCY potentiates KCl-induced umbilical artery vasoconstriction, in part by increasing Ca2+ influx in vascular smooth muscle cells via activation of Ca2+ channels. MLT significantly suppressed the vasoconstrictive effect of HCY, probably by scavenging ·OH arising from HCY autooxidation.
AB - Homocysteinemia is a major and independent risk factor for vascular disease. Oxidative stress is a possible mechanism for homocysteine (HCY)-induced vascular disease. Herein, we evaluated the antioxidant property of melatonin (MLT) in relation to the vasoconstrictive effect of HCY on the human umbilical artery. Helical umbilical arterial strips without endothelium were obtained at elective Cesarean delivery near term. Changes in potassium chloride (KCl)-induced vasoconstriction were measured. Arterial strips were treated with HCY (10 or 100 μM) plus FeSO4 (10 μM) alone or pretreated with a hydroxyl radical (·OH) scavenger, mannitol (20 mM), or MLT (1 or 10 μM). The effect of HCY on the response of arterial strips to external calcium (Ca2+) in the presence of KCl (20 mM) was determined. HCY plus FeSO4 potentiated KCl-induced vasoconstriction in a concentration-dependent manner; pretreatment with mannitol significantly reduced this vasospastic effect. HCY (100 μM) significantly augmented the contractile response to external Ca2+. MLT (10 μM) significantly suppressed the contractile response to external Ca2+. These results suggest that HCY potentiates KCl-induced umbilical artery vasoconstriction, in part by increasing Ca2+ influx in vascular smooth muscle cells via activation of Ca2+ channels. MLT significantly suppressed the vasoconstrictive effect of HCY, probably by scavenging ·OH arising from HCY autooxidation.
KW - Calcium
KW - Homocysteine
KW - Melatonin
KW - Umbilical artery
KW - Vasospasm
UR - http://www.scopus.com/inward/record.url?scp=0034805552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034805552&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2000.4211
DO - 10.1006/bbrc.2000.4211
M3 - Article
VL - 280
SP - 940
EP - 944
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -