The protective effect of melatonin against oxidant-induced free radical damage was examined in vitro. Lymphocytes, isolated from rat spleen, were incubated for 60 min with H2O2 (5 mM) or FeSO4 (20 μM), either alone or together with melatonin or glutathione at increasing concentrations from 0.1 to 2 mM. Lymphocytic malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) concentrations were measured as indices of induced membrane peroxidative damage. The level of lipid peroxidation products in lymphocytes increased significantly after the combination of H2O2 and FeSO4; that effect was abolished by both melatonin and glutathione. The inhibitory effect of melatonin on lipid peroxidation was directly correlated with the concentration of the indole in medium. Additionally, the effect of melatonin was compared with that of well-known endogenous antioxidant, glutathione.
|Original language||English (US)|
|Number of pages||5|
|Journal||International Journal of Thymology|
|Issue number||SUPPL. 1|
|State||Published - Jan 1 1996|
- lipid peroxidation
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