Meiosis-specific recombinase Dmc1 is a potent inhibitor of the Srs2 antirecombinase

J. Brooks Crickarda, Kyle Kaniecki, Youngho Kwon, Patrick Sung, Eric C. Greene

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Cross-over recombination products are a hallmark of meiosis because they are necessary for accurate chromosome segregation and they also allow for increased genetic diversity during sexual reproduction. However, cross-overs can also cause gross chromosomal rearrangements and are therefore normally down-regulated during mitotic growth. The mechanisms that enhance cross-over product formation upon entry into meiosis remain poorly understood. In Saccharomyces cerevisiae, the Superfamily 1 (Sf1) helicase Srs2, which is an ATP hydrolysis-dependent motor protein that actively dismantles recombination intermediates, promotes synthesisdependent strand annealing, the result of which is a reduction in cross-over recombination products. Here, we show that the meiosisspecific recombinase Dmc1 is a potent inhibitor of Srs2. Biochemical and single-molecule assays demonstrate that Dmc1 acts by inhibiting Srs2 ATP hydrolysis activity, which prevents the motor protein from undergoing ATP hydrolysis-dependent translocation on Dmc1- bound recombination intermediates. We propose a model in which Dmc1 helps contribute to cross-over formation during meiosis by antagonizing the antirecombinase activity of Srs2.

Original languageEnglish (US)
Pages (from-to)E10041-E10048
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number43
StatePublished - Oct 23 2018
Externally publishedYes


  • Dmc1
  • Homologous recombination
  • Meiosis
  • Rad51
  • Srs2

ASJC Scopus subject areas

  • General


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