Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system

Luis A. Medina, Sergio M. Calixto, Robert Klipper, Yang Li, William T Phillips, Beth Goins

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A method for delivering drugs to sites of disease extension in mediastinal nodes is described. Mediastinal node and lymphatic distributions were determined after intracavitary injection of the avidin/biotin-liposome system in normal rats. The effect of the injected dose on lymphatic targeting of liposomes after intraperitoneal injection of 99mTc-blue-biotin-liposomes and intrapleural injection of avidin, and vice versa, is presented. Scintigraphic imaging was used to follow the movement of 99mTc-blue-biotin- liposomes to determine the pharmacokinetics and organ uptake. Tissue biodistribution studies were performed 22 h after injection of the 99mTc-blue-biotin-liposomes. Results indicated that independent of the cavity in which each agent was injected, a dose of 5.0 mg of each agent results in higher mediastinal node targeting (8%-10% ID/Organ) as compared with the injection of a 0.5 mg dose (2%-5% ID/Organ, p < 0.05). Targeting of diaphragm and associated lymphatics was observed when 99mTc-blue- biotin-liposomes were injected in peritoneum and avidin in pleural space. In contrast, pleural, and pericardial lymphatic targeting was observed when 99mTc-blue-biotin-liposomes were injected in pleural space and avidin in peritoneum. Intracavitary injection of the avidin/biotin-liposome system could potentially be used for the delivery of prophylactic drugs that could reduce tumor metastasis and infection spread to mediastinal nodes.

Original languageEnglish (US)
Pages (from-to)207-224
Number of pages18
JournalJournal of Pharmaceutical Sciences
Volume95
Issue number1
DOIs
StatePublished - Jan 2006

Fingerprint

Avidin
Biotin
Liposomes
Injections
Peritoneum
Pharmacokinetics
Diaphragms
Diaphragm
Intraperitoneal Injections
Pharmaceutical Preparations
Rats
Tumors
Tissue
Neoplasm Metastasis
Imaging techniques
Infection

Keywords

  • Avidin-biotin system
  • Drug targeting
  • Liposomes
  • Lymphatic transport
  • Nanoparticles
  • Peritoneum
  • Pharmacokinetics
  • Pleural space
  • Scintigraphy
  • Technetium-99m

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Chemistry(all)
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science

Cite this

Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system. / Medina, Luis A.; Calixto, Sergio M.; Klipper, Robert; Li, Yang; Phillips, William T; Goins, Beth.

In: Journal of Pharmaceutical Sciences, Vol. 95, No. 1, 01.2006, p. 207-224.

Research output: Contribution to journalArticle

Medina, Luis A. ; Calixto, Sergio M. ; Klipper, Robert ; Li, Yang ; Phillips, William T ; Goins, Beth. / Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system. In: Journal of Pharmaceutical Sciences. 2006 ; Vol. 95, No. 1. pp. 207-224.
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