TY - JOUR
T1 - Mechanisms of bunyavirus virulence. Comparative pathogenesis of a virulent strain of La Crosse and an avirulent strain of Tahyna virus
AU - Janssen, R.
AU - Gonzalez-Scarano, F.
AU - Nathanson, N.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1984
Y1 - 1984
N2 - To analyze the mechanisms of virulence in the California serogroup bunyaviruses, the virulent La Crosse/original (LAC/original) strain was compared with the avirulent Tahyna/181-57 strain. In suckling mice, both viruses were lethal upon intracerebral injection but differed markedly in their neuroinvasiveness following subcutaneous injection; 20 and 20,000 plaque-forming units, respectively, were equivalent to 1 subcutaneous LD50. The sequential course of infection was followed after subcutaneous injection of 700 plaque-forming units; LAC/original replicated in striated muscle, caused a high titer plasma viremia, invaded the central nervous system, and killed all mice; the same dose of avirulent Tahyna/181-57 failed to replicate in extraneural tissues, did not invade the central nervous system, and caused no apparent illness. Immunofluorescent examination of peripheral and central nervous system tissues showed the same distinctions between virulent LAC/original virus. Paradoxically, after intracerebral injection of suckling or adult mice, Tahyna/181-57 virus killed more quickly than LAC/original. This difference was correlated with replication differences; Tahyna/181-57 multiplied marginally faster in the brain than did LAC/original virus.
AB - To analyze the mechanisms of virulence in the California serogroup bunyaviruses, the virulent La Crosse/original (LAC/original) strain was compared with the avirulent Tahyna/181-57 strain. In suckling mice, both viruses were lethal upon intracerebral injection but differed markedly in their neuroinvasiveness following subcutaneous injection; 20 and 20,000 plaque-forming units, respectively, were equivalent to 1 subcutaneous LD50. The sequential course of infection was followed after subcutaneous injection of 700 plaque-forming units; LAC/original replicated in striated muscle, caused a high titer plasma viremia, invaded the central nervous system, and killed all mice; the same dose of avirulent Tahyna/181-57 failed to replicate in extraneural tissues, did not invade the central nervous system, and caused no apparent illness. Immunofluorescent examination of peripheral and central nervous system tissues showed the same distinctions between virulent LAC/original virus. Paradoxically, after intracerebral injection of suckling or adult mice, Tahyna/181-57 virus killed more quickly than LAC/original. This difference was correlated with replication differences; Tahyna/181-57 multiplied marginally faster in the brain than did LAC/original virus.
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M3 - Article
C2 - 6708454
AN - SCOPUS:0021282443
VL - 50
SP - 447
EP - 455
JO - Laboratory Investigation
JF - Laboratory Investigation
SN - 0023-6837
IS - 4
ER -