TY - JOUR
T1 - Mechanism of the age-related decline in lymphocyte proliferation
T2 - Role of IL-2 production and protein synthesis
AU - Tak Cheung, H.
AU - Twu, Jr Swin
AU - Richardson, A.
N1 - Funding Information:
and a grant (AG 01548) from the National Institutes of Health.
Funding Information:
Acknowledgement-Thwiosr k was supported by a grant from the Graduate School, Illinois State University,
PY - 1983
Y1 - 1983
N2 - Although an age-related decline in mitogen-induced proliferation in spleen lymphocytes has been reported by numerous investigators, the molecular mechanism responsible is unknown. In this study, we compared the mitogen-induced proliferation, IL-2 production, and protein synthesis in spleen lymphocytes isolated from 4, 12, 20 and 30 month-old male Fischer F344 rats. IL-2 production by Con A-stimulated lymphocytes, as determined by the ability of the culture supernatants to support the growth of cultured T cells, declined over 72% between 4 and 30 months of age. This decline in IL-2 production paralleled a similar decrease in proliferation. Early protein synthesis by Con A-stimulated spleen lymphocytes was determined by measuring the incorporation of [3H]-valine into acid insoluble material, and this dropped 74% between 4 and 30 months of age. There was a strong correlation between the age-related decline in the three parameters tested. Based on these results, we propose that the age-related decline in protein synthesis may be the molecular basis for the similar decrease in IL-2 production and mitogenesis.
AB - Although an age-related decline in mitogen-induced proliferation in spleen lymphocytes has been reported by numerous investigators, the molecular mechanism responsible is unknown. In this study, we compared the mitogen-induced proliferation, IL-2 production, and protein synthesis in spleen lymphocytes isolated from 4, 12, 20 and 30 month-old male Fischer F344 rats. IL-2 production by Con A-stimulated lymphocytes, as determined by the ability of the culture supernatants to support the growth of cultured T cells, declined over 72% between 4 and 30 months of age. This decline in IL-2 production paralleled a similar decrease in proliferation. Early protein synthesis by Con A-stimulated spleen lymphocytes was determined by measuring the incorporation of [3H]-valine into acid insoluble material, and this dropped 74% between 4 and 30 months of age. There was a strong correlation between the age-related decline in the three parameters tested. Based on these results, we propose that the age-related decline in protein synthesis may be the molecular basis for the similar decrease in IL-2 production and mitogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0021061964&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021061964&partnerID=8YFLogxK
U2 - 10.1016/0531-5565(83)90024-4
DO - 10.1016/0531-5565(83)90024-4
M3 - Article
C2 - 6609081
AN - SCOPUS:0021061964
VL - 18
SP - 451
EP - 460
JO - Experimental Gerontology
JF - Experimental Gerontology
SN - 0531-5565
IS - 6
ER -