Mechanical regulation of bone homeostasis: Effects of cyclic pressure on bone cell function in vitro

Jiro Nagatomi, Bernard P. Arulanandam, Dennis W. Metzger, Alain Meunier, Rena Bizios

Research output: Contribution to journalConference articlepeer-review


The present in vitro study exposed rat osteoblasts and osteoclast-precursors in bone marrow cell populations to controlled regimes of cyclic pressure and examined various cell functions that are pertinent to bone homeostasis. The results provided evidence that osteoblasts are sensitive to the frequency of the applied cyclic pressure stimulus. Specifically, compared to controls (static conditions) and cells exposed to cyclic pressure at 0.25 Hz frequency, osteoblast proliferation was significantly (p < 0.05) lower, but alkaline phosphatase mRNA expression and enzyme activity were enhanced, only when these cells were exposed to cyclic pressure at 1.0 Hz frequency for 1 hour daily for 5 consecutive days. Furthermore, the results of the present study demonstrated that the timing of application of the cyclic pressure was critical for osteoclastic cell formation from precursors in bone marrow. Exposure of bone marrow cells to cyclic pressure immediately upon harvesting led to decreased formation of osteoclastic cells from their precursors; in contrast, the number of osteoclastic cells was not affected when the cyclic pressure was applied after 7 days of culture under static conditions.

Original languageEnglish (US)
Pages (from-to)2981-2982
Number of pages2
JournalAnnual Reports of the Research Reactor Institute, Kyoto University
StatePublished - 2001
Externally publishedYes
Event23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey
Duration: Oct 25 2001Oct 28 2001

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Mechanical Engineering


Dive into the research topics of 'Mechanical regulation of bone homeostasis: Effects of cyclic pressure on bone cell function in vitro'. Together they form a unique fingerprint.

Cite this