Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy

Albert J. Jin, Kondury Prasad, Paul D. Smith, Eileen M. Lafer, Ralph Nossal

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Using a new scheme based on atomic force microscopy (AFM), we investigate mechanical properties of clathrin-coated vesicles (CCVs). CCVs are multicomponent protein and lipid complexes of ∼100 nm diameter that are implicated in many essential cell-trafficking processes. Our AFM imaging resolves clathrin lattice polygons and provides height deformation in quantitative response to AFM-substrate compression force. We model CCVs as multilayered elastic spherical shells and, from AFM measurements, estimate their bending rigidity to be 285 ± 30 kBT, i.e., ∼20 times that of either the outer clathrin cage or inner vesicle membrane. Further analysis reveals a flexible coupling between the clathrin coat and the membrane, a structural property whose modulation may affect vesicle biogenesis and cellular function.

Original languageEnglish (US)
Pages (from-to)3333-3344
Number of pages12
JournalBiophysical Journal
Volume90
Issue number9
DOIs
StatePublished - May 2006

ASJC Scopus subject areas

  • Biophysics

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    Jin, A. J., Prasad, K., Smith, P. D., Lafer, E. M., & Nossal, R. (2006). Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy. Biophysical Journal, 90(9), 3333-3344. https://doi.org/10.1529/biophysj.105.068742