Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy

Albert J. Jin, Kondury Prasad, Paul D. Smith, Eileen M Lafer, Ralph Nossal

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Using a new scheme based on atomic force microscopy (AFM), we investigate mechanical properties of clathrin-coated vesicles (CCVs). CCVs are multicomponent protein and lipid complexes of ∼100 nm diameter that are implicated in many essential cell-trafficking processes. Our AFM imaging resolves clathrin lattice polygons and provides height deformation in quantitative response to AFM-substrate compression force. We model CCVs as multilayered elastic spherical shells and, from AFM measurements, estimate their bending rigidity to be 285 ± 30 kBT, i.e., ∼20 times that of either the outer clathrin cage or inner vesicle membrane. Further analysis reveals a flexible coupling between the clathrin coat and the membrane, a structural property whose modulation may affect vesicle biogenesis and cellular function.

Original languageEnglish (US)
Pages (from-to)3333-3344
Number of pages12
JournalBiophysical Journal
Volume90
Issue number9
DOIs
StatePublished - May 2006

Fingerprint

Clathrin-Coated Vesicles
Atomic Force Microscopy
Elasticity
Clathrin
Membranes
Lipids
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy. / Jin, Albert J.; Prasad, Kondury; Smith, Paul D.; Lafer, Eileen M; Nossal, Ralph.

In: Biophysical Journal, Vol. 90, No. 9, 05.2006, p. 3333-3344.

Research output: Contribution to journalArticle

Jin, Albert J. ; Prasad, Kondury ; Smith, Paul D. ; Lafer, Eileen M ; Nossal, Ralph. / Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy. In: Biophysical Journal. 2006 ; Vol. 90, No. 9. pp. 3333-3344.
@article{61b01d294be44a8d91e58d5f01c58045,
title = "Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy",
abstract = "Using a new scheme based on atomic force microscopy (AFM), we investigate mechanical properties of clathrin-coated vesicles (CCVs). CCVs are multicomponent protein and lipid complexes of ∼100 nm diameter that are implicated in many essential cell-trafficking processes. Our AFM imaging resolves clathrin lattice polygons and provides height deformation in quantitative response to AFM-substrate compression force. We model CCVs as multilayered elastic spherical shells and, from AFM measurements, estimate their bending rigidity to be 285 ± 30 kBT, i.e., ∼20 times that of either the outer clathrin cage or inner vesicle membrane. Further analysis reveals a flexible coupling between the clathrin coat and the membrane, a structural property whose modulation may affect vesicle biogenesis and cellular function.",
author = "Jin, {Albert J.} and Kondury Prasad and Smith, {Paul D.} and Lafer, {Eileen M} and Ralph Nossal",
year = "2006",
month = "5",
doi = "10.1529/biophysj.105.068742",
language = "English (US)",
volume = "90",
pages = "3333--3344",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "9",

}

TY - JOUR

T1 - Measuring the elasticity of clathrin-coated vesicles via atomic force microscopy

AU - Jin, Albert J.

AU - Prasad, Kondury

AU - Smith, Paul D.

AU - Lafer, Eileen M

AU - Nossal, Ralph

PY - 2006/5

Y1 - 2006/5

N2 - Using a new scheme based on atomic force microscopy (AFM), we investigate mechanical properties of clathrin-coated vesicles (CCVs). CCVs are multicomponent protein and lipid complexes of ∼100 nm diameter that are implicated in many essential cell-trafficking processes. Our AFM imaging resolves clathrin lattice polygons and provides height deformation in quantitative response to AFM-substrate compression force. We model CCVs as multilayered elastic spherical shells and, from AFM measurements, estimate their bending rigidity to be 285 ± 30 kBT, i.e., ∼20 times that of either the outer clathrin cage or inner vesicle membrane. Further analysis reveals a flexible coupling between the clathrin coat and the membrane, a structural property whose modulation may affect vesicle biogenesis and cellular function.

AB - Using a new scheme based on atomic force microscopy (AFM), we investigate mechanical properties of clathrin-coated vesicles (CCVs). CCVs are multicomponent protein and lipid complexes of ∼100 nm diameter that are implicated in many essential cell-trafficking processes. Our AFM imaging resolves clathrin lattice polygons and provides height deformation in quantitative response to AFM-substrate compression force. We model CCVs as multilayered elastic spherical shells and, from AFM measurements, estimate their bending rigidity to be 285 ± 30 kBT, i.e., ∼20 times that of either the outer clathrin cage or inner vesicle membrane. Further analysis reveals a flexible coupling between the clathrin coat and the membrane, a structural property whose modulation may affect vesicle biogenesis and cellular function.

UR - http://www.scopus.com/inward/record.url?scp=33646155956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646155956&partnerID=8YFLogxK

U2 - 10.1529/biophysj.105.068742

DO - 10.1529/biophysj.105.068742

M3 - Article

C2 - 16473913

AN - SCOPUS:33646155956

VL - 90

SP - 3333

EP - 3344

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 9

ER -