MEASUREMENT OF THE RATE OF GLUCOSE UTILIZATION BY RAT BRAIN IN VIVO

R. A. Hawkins; A. L. Miller; Jill E. Cremer; R. L. Veech

doi:10.1111/j.1471-4159.1974.tb10743.x

MEASUREMENT OF THE RATE OF GLUCOSE UTILIZATION BY RAT BRAIN IN VIVO

R. A. Hawkins, A. L. Miller, Jill E. Cremer, R. L. Veech

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Abstract— A method is described by which the rate of glucose utilization by whole brain of conscious rats may be measured. The basis is the uptake of ¹⁴C derived front [2‐¹⁴C] glucose into the acid‐soluble metabolite pool of brain. Catheters are placed in the femoral artery and vein under light ether anesthesia. After full recovery of consciousness a single intravenous injection of [2‐¹⁴C] glucose is given and arterial blood samples taken at intervals. Simultaneous with the last sample the brain is removed and frozen within 1 s. The accumulation of ¹⁴C into the acid‐soluble metabilite pool is measured and the rate of glucose utilization is calculated according to the equation: (Formula Presented.) The integral is calculated from the plasma glucose specific activity curve and evidence is presented to justify this procedure. The rate of glucose utilization measured by this method was 0·62 μmol/min per g in conscious rats and 0·28 μmol/min per g in sodium pentobarbital anesthetized rats.

Original language	English (US)
Pages (from-to)	917-923
Number of pages	7
Journal	Journal of Neurochemistry
Volume	23
Issue number	5
DOIs	https://doi.org/10.1111/j.1471-4159.1974.tb10743.x
State	Published - Nov 1974
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1974.tb10743.x

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title = "MEASUREMENT OF THE RATE OF GLUCOSE UTILIZATION BY RAT BRAIN IN VIVO",

abstract = "Abstract— A method is described by which the rate of glucose utilization by whole brain of conscious rats may be measured. The basis is the uptake of 14C derived front [2‐14C] glucose into the acid‐soluble metabolite pool of brain. Catheters are placed in the femoral artery and vein under light ether anesthesia. After full recovery of consciousness a single intravenous injection of [2‐14C] glucose is given and arterial blood samples taken at intervals. Simultaneous with the last sample the brain is removed and frozen within 1 s. The accumulation of 14C into the acid‐soluble metabilite pool is measured and the rate of glucose utilization is calculated according to the equation: (Formula Presented.) The integral is calculated from the plasma glucose specific activity curve and evidence is presented to justify this procedure. The rate of glucose utilization measured by this method was 0·62 μmol/min per g in conscious rats and 0·28 μmol/min per g in sodium pentobarbital anesthetized rats.",

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year = "1974",

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AU - Miller, A. L.

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N2 - Abstract— A method is described by which the rate of glucose utilization by whole brain of conscious rats may be measured. The basis is the uptake of 14C derived front [2‐14C] glucose into the acid‐soluble metabolite pool of brain. Catheters are placed in the femoral artery and vein under light ether anesthesia. After full recovery of consciousness a single intravenous injection of [2‐14C] glucose is given and arterial blood samples taken at intervals. Simultaneous with the last sample the brain is removed and frozen within 1 s. The accumulation of 14C into the acid‐soluble metabilite pool is measured and the rate of glucose utilization is calculated according to the equation: (Formula Presented.) The integral is calculated from the plasma glucose specific activity curve and evidence is presented to justify this procedure. The rate of glucose utilization measured by this method was 0·62 μmol/min per g in conscious rats and 0·28 μmol/min per g in sodium pentobarbital anesthetized rats.

AB - Abstract— A method is described by which the rate of glucose utilization by whole brain of conscious rats may be measured. The basis is the uptake of 14C derived front [2‐14C] glucose into the acid‐soluble metabolite pool of brain. Catheters are placed in the femoral artery and vein under light ether anesthesia. After full recovery of consciousness a single intravenous injection of [2‐14C] glucose is given and arterial blood samples taken at intervals. Simultaneous with the last sample the brain is removed and frozen within 1 s. The accumulation of 14C into the acid‐soluble metabilite pool is measured and the rate of glucose utilization is calculated according to the equation: (Formula Presented.) The integral is calculated from the plasma glucose specific activity curve and evidence is presented to justify this procedure. The rate of glucose utilization measured by this method was 0·62 μmol/min per g in conscious rats and 0·28 μmol/min per g in sodium pentobarbital anesthetized rats.

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MEASUREMENT OF THE RATE OF GLUCOSE UTILIZATION BY RAT BRAIN IN VIVO

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