Abstract
Transforming growth factor-beta-1 (TGF-b1) and Endothelin-1 (ET1) have been implicated in the pathogenesis of diabetic renal disease. To evaluate if renal production of TGF-b1 and ET-1 is enhanced in diabetic patients, we studied diabetic (D) and non-diabetic patients (N) with varying degrees of renal function undergoing elective cardiac cathetehzation. A thermodilution catheter was inserted into the left renal vein for blood sampling and measurement of renal blood flow (RBF). Renal vein (RV) and aortic (AO) plasma levels of TGF-b1 and ET-1 levels were measured by sandwich ELISA and RIA, respectively. 22 patients were enrolled, 11 N and 11 D ( all NIDDM, 3 taking insulin). Renal vein (RV) levels of TGF-bt were almost two-fold greater in D vs N (15.6 ±1.9 vs 8.4 ±0.8 ng/ml, respectively, P=0.003) but not different in the aortic (Ao) samples (14.2 ±1.9 vs 13.4 ±1.1 ng/ml). 7/11 of D had greater renal vein levels than aortic levels, indicating renal production of TGF-b1 whereas all 11 of N had lower renal vein levels as compared to aortic levels, indicating renal clearance of TGF-b1. Urinary levels of TGF-b1 were significantly elevated in D vs N (2.59 ±0.94 vs 0.23 ± 0.09 ng/mg créât, P=0.01 ), demonstrating that impaired renal clearance was not the explanation for elevated renal vein levels. Renal vein TGF-b1 levels were inversely correlated with GFR in both D and N. Both aortic and renal vein levels of ET-1 were significantly greater in D than in N, (Ao: 42 ±4.3 vs 29.3 ±1.5, P=0.01; RV: 44.1 ±4.4 vs 28.8 ±1.5 fM, P<0.01). We conclude that patients with NIDDM manifest net renal production of TGF-b1 and have elevated systemic levels of ET-1 which may contribute to the development of diabetic nephropathy.
| Original language | English (US) |
|---|---|
| Journal | Journal of Investigative Medicine |
| Volume | 44 |
| Issue number | 3 |
| State | Published - Jan 1 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Measurement of renal production of TGF-B1 and endothelin-1 in diabetic and non-diabetic patients. / Alzahabi, B.; Kapoor, S.; McGowan, T.; Kumik, B.; Kurnik, P.; Ziyadeh, F. N.; Weisberg, L.; Sharma, Kumar.
In: Journal of Investigative Medicine, Vol. 44, No. 3, 01.01.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Measurement of renal production of TGF-B1 and endothelin-1 in diabetic and non-diabetic patients
AU - Alzahabi, B.
AU - Kapoor, S.
AU - McGowan, T.
AU - Kumik, B.
AU - Kurnik, P.
AU - Ziyadeh, F. N.
AU - Weisberg, L.
AU - Sharma, Kumar
PY - 1996/1/1
Y1 - 1996/1/1
N2 - Transforming growth factor-beta-1 (TGF-b1) and Endothelin-1 (ET1) have been implicated in the pathogenesis of diabetic renal disease. To evaluate if renal production of TGF-b1 and ET-1 is enhanced in diabetic patients, we studied diabetic (D) and non-diabetic patients (N) with varying degrees of renal function undergoing elective cardiac cathetehzation. A thermodilution catheter was inserted into the left renal vein for blood sampling and measurement of renal blood flow (RBF). Renal vein (RV) and aortic (AO) plasma levels of TGF-b1 and ET-1 levels were measured by sandwich ELISA and RIA, respectively. 22 patients were enrolled, 11 N and 11 D ( all NIDDM, 3 taking insulin). Renal vein (RV) levels of TGF-bt were almost two-fold greater in D vs N (15.6 ±1.9 vs 8.4 ±0.8 ng/ml, respectively, P=0.003) but not different in the aortic (Ao) samples (14.2 ±1.9 vs 13.4 ±1.1 ng/ml). 7/11 of D had greater renal vein levels than aortic levels, indicating renal production of TGF-b1 whereas all 11 of N had lower renal vein levels as compared to aortic levels, indicating renal clearance of TGF-b1. Urinary levels of TGF-b1 were significantly elevated in D vs N (2.59 ±0.94 vs 0.23 ± 0.09 ng/mg créât, P=0.01 ), demonstrating that impaired renal clearance was not the explanation for elevated renal vein levels. Renal vein TGF-b1 levels were inversely correlated with GFR in both D and N. Both aortic and renal vein levels of ET-1 were significantly greater in D than in N, (Ao: 42 ±4.3 vs 29.3 ±1.5, P=0.01; RV: 44.1 ±4.4 vs 28.8 ±1.5 fM, P<0.01). We conclude that patients with NIDDM manifest net renal production of TGF-b1 and have elevated systemic levels of ET-1 which may contribute to the development of diabetic nephropathy.
AB - Transforming growth factor-beta-1 (TGF-b1) and Endothelin-1 (ET1) have been implicated in the pathogenesis of diabetic renal disease. To evaluate if renal production of TGF-b1 and ET-1 is enhanced in diabetic patients, we studied diabetic (D) and non-diabetic patients (N) with varying degrees of renal function undergoing elective cardiac cathetehzation. A thermodilution catheter was inserted into the left renal vein for blood sampling and measurement of renal blood flow (RBF). Renal vein (RV) and aortic (AO) plasma levels of TGF-b1 and ET-1 levels were measured by sandwich ELISA and RIA, respectively. 22 patients were enrolled, 11 N and 11 D ( all NIDDM, 3 taking insulin). Renal vein (RV) levels of TGF-bt were almost two-fold greater in D vs N (15.6 ±1.9 vs 8.4 ±0.8 ng/ml, respectively, P=0.003) but not different in the aortic (Ao) samples (14.2 ±1.9 vs 13.4 ±1.1 ng/ml). 7/11 of D had greater renal vein levels than aortic levels, indicating renal production of TGF-b1 whereas all 11 of N had lower renal vein levels as compared to aortic levels, indicating renal clearance of TGF-b1. Urinary levels of TGF-b1 were significantly elevated in D vs N (2.59 ±0.94 vs 0.23 ± 0.09 ng/mg créât, P=0.01 ), demonstrating that impaired renal clearance was not the explanation for elevated renal vein levels. Renal vein TGF-b1 levels were inversely correlated with GFR in both D and N. Both aortic and renal vein levels of ET-1 were significantly greater in D than in N, (Ao: 42 ±4.3 vs 29.3 ±1.5, P=0.01; RV: 44.1 ±4.4 vs 28.8 ±1.5 fM, P<0.01). We conclude that patients with NIDDM manifest net renal production of TGF-b1 and have elevated systemic levels of ET-1 which may contribute to the development of diabetic nephropathy.
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M3 - Article
VL - 44
JO - Journal of Investigative Medicine
JF - Journal of Investigative Medicine
SN - 1081-5589
IS - 3
ER -