@inbook{eb8e0d1444a64cf19167b4b1aee2f11d,
title = "Measurement of Kinetic Isotope Effects in an Enzyme-Catalyzed Reaction by Continuous-Flow Mass Spectrometry",
abstract = "Kinetic isotope effects (KIEs) provide powerful probes of the mechanisms of enzyme-catalyzed reactions. In this chapter, we describe the use of continuous-flow mass spectrometry to determine the deuterium KIE for the enzyme N-acetylpolyamine oxidase based on the ratio of labeled and unlabeled products in mass spectra of whole reaction mixtures.",
keywords = "Amine oxidase, Continuous flow, Enzyme, Flavoprotein, Isotope effect, Mass spectrometry, Polyamine oxidase",
author = "Roberts, {Kenneth M.} and Fitzpatrick, {Paul F.}",
note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",
year = "2017",
doi = "10.1016/bs.mie.2017.07.001",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "149--161",
booktitle = "Methods in Enzymology",
address = "United States",
}