Measurement of human immunodeficiency virus type 1 plasma virus load based on reverse transcriptase (RT) activity: Evidence of variabilities in levels of virion-associated RT

J. Gerardo García Lerma, Shinji Yamamoto, María Gómez-Cano, Vincent Soriano, Timothy A. Green, Michael P. Busch, Thomas M. Folks, Walid Heneine

    Research output: Contribution to journalArticlepeer-review

    37 Scopus citations

    Abstract

    Virus load based on levels of functional reverse transcriptase (RT) was measured in plasma from 50 human immunodeficiency virus (HIV) type 1-infected persons, in 87 samples from 10 HIV-1 seroconversion panels, and in 100 uninfected persons by use of Amp-RT, an ultrasensitive RT assay. Of the 50 clinical samples, 38 (76%) were Amp-RT positive, while all uninfected controls were negative. Pearson's correlation coefficient of RNA and RT levels was .73 for all samples, .86 for seroconversion samples, and .49 for clinical samples. Calculated ratios of RT activity to virion RNA varied widely during both early and late stages of infection. Mean RT:RNA ratios in 8 seroconversion panels and in 12 (34.3%) of 35 individual clinical samples were significantly lower than the ratio for a reference virus. However, ratios were stable in individual seroconversions over time. These data demonstrate that RT activity can be used to quantitate plasma virus load and provide evidence of different levels of virion-associated RT among HIV-1- infected persons.

    Original languageEnglish (US)
    Pages (from-to)1221-1229
    Number of pages9
    JournalJournal of Infectious Diseases
    Volume177
    Issue number5
    DOIs
    StatePublished - 1998

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Infectious Diseases

    Fingerprint Dive into the research topics of 'Measurement of human immunodeficiency virus type 1 plasma virus load based on reverse transcriptase (RT) activity: Evidence of variabilities in levels of virion-associated RT'. Together they form a unique fingerprint.

    Cite this