TY - JOUR
T1 - Maternal Nutrient Restriction Upregulates Growth Signaling Pathways in the Cotyledonary Artery of Cow Placentomes
AU - Zhu, M. J.
AU - Du, M.
AU - Hess, B. W.
AU - Means, W. J.
AU - Nathanielsz, P. W.
AU - Ford, S. P.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/4
Y1 - 2007/4
N2 - This study evaluated the role of MAPK/ERK1/2 and/or PI3-K/Akt signaling pathways in modulating bovine placentomal vascularity in response to maternal nutrient restriction. Beef cows were randomly assigned to control fed (Control, n = 15, 100% of requirements) or nutrient restricted (NR, n = 15, 50% requirements) diets from day 30 to day 125 of gestation. Ten cows from each dietary group were necropsied on day 125 (∼45% gestation), and the remaining cows in each diet group were then fed control diets and necropsied on day 250 (∼90% gestation). At day 125 of gestation, NR cows exhibited increased (P = 0.06) COT vascularity, improved (P < 0.05) placentome efficiency (fetal weight/placentomal weight), and increased (P < 0.05) phosphorylated Akt and ERK1/2 in COT arteries compared to Control cows. By day 250, however, treatment differences in COT vascularity and phosphorylated Akt and ERK1/2 in COT arteries were lost. On both gestational days, no treatment difference was observed in the levels of phosphorylated Akt or ERK1/2 in CAR arteries. CAR vascularity was similar across treatment on day 125, but tended to be greater (P < 0.10) in NR than Control cows on day 250. These data suggest that conceptuses react to an early gestational nutrient restriction by up-regulating COT growth signaling pathways associated with angiogenesis, and that these compensations do not persist to term.
AB - This study evaluated the role of MAPK/ERK1/2 and/or PI3-K/Akt signaling pathways in modulating bovine placentomal vascularity in response to maternal nutrient restriction. Beef cows were randomly assigned to control fed (Control, n = 15, 100% of requirements) or nutrient restricted (NR, n = 15, 50% requirements) diets from day 30 to day 125 of gestation. Ten cows from each dietary group were necropsied on day 125 (∼45% gestation), and the remaining cows in each diet group were then fed control diets and necropsied on day 250 (∼90% gestation). At day 125 of gestation, NR cows exhibited increased (P = 0.06) COT vascularity, improved (P < 0.05) placentome efficiency (fetal weight/placentomal weight), and increased (P < 0.05) phosphorylated Akt and ERK1/2 in COT arteries compared to Control cows. By day 250, however, treatment differences in COT vascularity and phosphorylated Akt and ERK1/2 in COT arteries were lost. On both gestational days, no treatment difference was observed in the levels of phosphorylated Akt or ERK1/2 in CAR arteries. CAR vascularity was similar across treatment on day 125, but tended to be greater (P < 0.10) in NR than Control cows on day 250. These data suggest that conceptuses react to an early gestational nutrient restriction by up-regulating COT growth signaling pathways associated with angiogenesis, and that these compensations do not persist to term.
KW - Akt and ERK1/2 phosphorylation
KW - Cow
KW - Nutrient restriction
KW - Placentome vascularity
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U2 - 10.1016/j.placenta.2006.04.005
DO - 10.1016/j.placenta.2006.04.005
M3 - Article
C2 - 16822544
AN - SCOPUS:33947729127
VL - 28
SP - 361
EP - 368
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 4
ER -