Mass spectrometric analysis of platelet-activating factor after isolation by solid-phase extraction and direct derivatization with pentafluorobenzoic anhydride

Susan T. Weintraub, Rajiv K. Satsangi, Eugene A. Sprague, Thomas J. Prihoda, R. Neal Pinckard

Research output: Contribution to journalLetterpeer-review

6 Scopus citations

Abstract

Platelet-activating factor is the term used to denote a class of extremely potent lipid mediators that consist predominantly of 1-O-alkyl- and 1-O-acyl-2-acetyl-sn-glycero-3-phosphocholines. A method has been devised for rapid isolation of these acetylated phospholipids by solid-phase extraction prior to direct derivatization with pentafluorobenzoic anhydride and analysis by gas chromatography (GC)/electron-capture mass spectrometry. Recovery through the entire method (lipid isolation, derivatization, and purification) typically ranged from 70% to 85%. Using the direct derivatization procedure described here, the practical limit of detection for each of the standard alkyl- and acyl-platelet-activating factor homologs was 1 fmol injected into the GC. Results from the application of the method to the analysis of alkyl and acyl homologs of platelet-activating factor isolated from stimulated human umbilical vein endothelial cells are presented, exhibiting excellent accuracy and precision for a wide range of tissue levels of this class of potent autacoids.

Original languageEnglish (US)
Pages (from-to)176-181
Number of pages6
JournalJournal of the American Society for Mass Spectrometry
Volume11
Issue number2
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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