Mapping of BRCT1 domain of BRCA1 with chromatin unfolding activity

Qi Nong Ye, Yan Fen Hu, Hong Jun Zhong, Rong Li, Cui Fen Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Breast cancer susceptibility gene 1(BRCA1) plays an important role in breast cancer development and progression. BRCA1 encodes a 1863-amino acid protein with two BRCA1 C-terminal (BRCT) domains at its C-terminus, BRCT1 and BRCT2. Many cancer-predisposing mutations are located in the BRCT domains, which have been shown to induce chromatin unfolding by use of an approach that allows visualization of large-scale chromatin structure through lac repressor/lac operator recognition. To map the important region of BRCT domain (amino acid residues 1642-1736), six deletion mutant constructs were made. The chromatin structure assay showed that amino acid residues 1691-1721 are involved in the induction of chromatin unfolding. To further localize the critical amino acid residues, ten alanine scanning mutant constructs were made. The chromatin structure assay demonstrated that the 1707IAGGK1711 region is critical for the chromatin unfolding activity. Based on the mapped important region, Blast analysis identified a novel homologous protein. Mapping of the BRCT1 domain may aid in the presymptomatic risk assessment and provide a valuable tool for further study on the BRCT1 structure and function.

Original languageEnglish (US)
Pages (from-to)656-661
Number of pages6
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume18
Issue number6
StatePublished - Nov 2002

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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