Mapping immunodominant antigens and H-2-linked antibody responses in mice urogenitally infected with Chlamydia muridarum

Hao Zeng, Shuping Hou, Siqi Gong, Xiaohua Dong, Quanming Zou, Guangming Zhong

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To identify immunodominant antigens and MHC-restricted antibody responses, seven different strains of mice were intravaginally infected with Chlamydia muridarum and compared for antibody responses to 257 C. muridarum proteins. The 7 strains of mice recognized a total of 109 proteins as antigens, of which, 5 antigens (TC0660, TC0727, TC0828, TC0726 & TC0268) were each recognized by 60% or more mice from each mouse strain and thus designated as immunodominant antigens. Furthermore, antibody responses to 19 other antigens displayed strong associations with mouse H-2 haplotypes, including 6 antigens (TC0480, TC0912, TC0229, TCA04, TC0289 & TC0892) whose antibody responses were linked to H-2 b, 8 (TC0035, TC0387, TC0052, TC0781, TC0373, TC0117, TC0066 & TC0396) to H-2 d and 5 (TC0512, TC0177, TC0589, TC0794 & TC0596) to H-2 k haplotypes respectively. Interestingly, H-2 b was negatively associated with antibody responses to most of the antigens that were positively linked to H-2 d or H-2 k haplotypes. These results by mapping Chlamydia trachomatis antigens commonly recognized by mice with different strain background and H-2 genes and revealing antigen association with H-2 haplotypes have provided important information for developing chlamydial subunit vaccines and understanding chlamydial pathogenesis.

Original languageEnglish (US)
Pages (from-to)659-665
Number of pages7
JournalMicrobes and Infection
Volume14
Issue number7-8
DOIs
StatePublished - Jul 1 2012

Keywords

  • Chlamydia muridarum
  • H-2-restricted antibody responses
  • Immunodominant antigens
  • Mouse urogenital tract infection

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Mapping immunodominant antigens and H-2-linked antibody responses in mice urogenitally infected with Chlamydia muridarum'. Together they form a unique fingerprint.

  • Cite this