Abstract
Identification of protective determinants from microbial proteins is a necessary step in the rational design of subunit vaccines. We have previously used a synthetic peptide scan (Pepscan) assay to map a panel of eight neutralizing monoclonal antibodies (mAb; designated as C1.1 to C1.8) to a common motif sequence from Chlamydia trachomatis. In the present study, five of the eight mAbs were used to screen phage random peptide libraries. mAbs C1.1 and C1.3 selected a motif sequence of G-L-X-N-D from a pIII-based phage random peptide library and a motif sequence of G-X-X-N-D from a pVIII-based random peptide library while mAbs C1.6 to C1.8 failed to select recognizable motifs from either of the phage libraries. However, C1.6 to C1.8 bound to the same motif sequence displayed on phage when the appropriate conformational constraints were imposed onto the motif sequence. Thus the specificity of the mAbs identified on Pepscan assays correlates with the mAbs' dependence on local epitope constraints displayed on the phage surface.
Original language | English (US) |
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Pages (from-to) | 71-76 |
Number of pages | 6 |
Journal | Journal of Industrial Microbiology and Biotechnology |
Volume | 19 |
Issue number | 1 |
DOIs | |
State | Published - 1997 |
Externally published | Yes |
Keywords
- Chlamydia trachomatis
- Epitope mapping
- Phage random peptide libraries
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Bioengineering
- Biotechnology