Mapping epitopes of neutralizing monoclonal antibodies using phage random peptide libraries

G. Zhong, J. D. Berry, S. Choukri

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Identification of protective determinants from microbial proteins is a necessary step in the rational design of subunit vaccines. We have previously used a synthetic peptide scan (Pepscan) assay to map a panel of eight neutralizing monoclonal antibodies (mAb; designated as C1.1 to C1.8) to a common motif sequence from Chlamydia trachomatis. In the present study, five of the eight mAbs were used to screen phage random peptide libraries. mAbs C1.1 and C1.3 selected a motif sequence of G-L-X-N-D from a pIII-based phage random peptide library and a motif sequence of G-X-X-N-D from a pVIII-based random peptide library while mAbs C1.6 to C1.8 failed to select recognizable motifs from either of the phage libraries. However, C1.6 to C1.8 bound to the same motif sequence displayed on phage when the appropriate conformational constraints were imposed onto the motif sequence. Thus the specificity of the mAbs identified on Pepscan assays correlates with the mAbs' dependence on local epitope constraints displayed on the phage surface.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalJournal of Industrial Microbiology and Biotechnology
Volume19
Issue number1
DOIs
StatePublished - Nov 11 1997
Externally publishedYes

Keywords

  • Chlamydia trachomatis
  • Epitope mapping
  • Phage random peptide libraries

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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