Abstract
Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1422-1429 |
| Number of pages | 8 |
| Journal | Journal of Magnetic Resonance Imaging |
| Volume | 34 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2011 |
| Externally published | Yes |
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Keywords
- high resolution imaging
- magnetic resonance imaging
- MEMRI
- MRI microscopy
- RCS
- retinal degeneration
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
Cite this
Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas. / Nair, Govind; Pardue, MacHelle T.; Kim, Moon; Duong, Timothy Q.
In: Journal of Magnetic Resonance Imaging, Vol. 34, No. 6, 12.2011, p. 1422-1429.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas
AU - Nair, Govind
AU - Pardue, MacHelle T.
AU - Kim, Moon
AU - Duong, Timothy Q.
PY - 2011/12
Y1 - 2011/12
N2 - Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.
AB - Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.
KW - high resolution imaging
KW - magnetic resonance imaging
KW - MEMRI
KW - MRI microscopy
KW - RCS
KW - retinal degeneration
UR - http://www.scopus.com/inward/record.url?scp=81755183117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=81755183117&partnerID=8YFLogxK
U2 - 10.1002/jmri.22719
DO - 10.1002/jmri.22719
M3 - Article
C2 - 21964629
AN - SCOPUS:81755183117
VL - 34
SP - 1422
EP - 1429
JO - Journal of Magnetic Resonance Imaging
JF - Journal of Magnetic Resonance Imaging
SN - 1053-1807
IS - 6
ER -