Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas

Govind Nair, MacHelle T. Pardue, Moon Kim, Timothy Q. Duong

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.

Original languageEnglish (US)
Pages (from-to)1422-1429
Number of pages8
JournalJournal of Magnetic Resonance Imaging
Volume34
Issue number6
DOIs
StatePublished - Dec 2011
Externally publishedYes

Fingerprint

Manganese
Blood Vessels
Retina
Magnetic Resonance Imaging
Retinal Vessels
Gadolinium DTPA
Blood-Retinal Barrier
Retinal Diseases
Retinal Pigment Epithelium
Ganglia
Histology
Injections
manganese chloride

Keywords

  • high resolution imaging
  • magnetic resonance imaging
  • MEMRI
  • MRI microscopy
  • RCS
  • retinal degeneration

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas. / Nair, Govind; Pardue, MacHelle T.; Kim, Moon; Duong, Timothy Q.

In: Journal of Magnetic Resonance Imaging, Vol. 34, No. 6, 12.2011, p. 1422-1429.

Research output: Contribution to journalArticle

Nair, Govind ; Pardue, MacHelle T. ; Kim, Moon ; Duong, Timothy Q. / Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas. In: Journal of Magnetic Resonance Imaging. 2011 ; Vol. 34, No. 6. pp. 1422-1429.
@article{f302c9de36144644b3e97cc96b017bef,
title = "Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas",
abstract = "Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.",
keywords = "high resolution imaging, magnetic resonance imaging, MEMRI, MRI microscopy, RCS, retinal degeneration",
author = "Govind Nair and Pardue, {MacHelle T.} and Moon Kim and Duong, {Timothy Q.}",
year = "2011",
month = "12",
doi = "10.1002/jmri.22719",
language = "English (US)",
volume = "34",
pages = "1422--1429",
journal = "Journal of Magnetic Resonance Imaging",
issn = "1053-1807",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Manganese-enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas

AU - Nair, Govind

AU - Pardue, MacHelle T.

AU - Kim, Moon

AU - Duong, Timothy Q.

PY - 2011/12

Y1 - 2011/12

N2 - Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.

AB - Purpose: To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods: Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results: MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA-which does not cross the blood-retinal barrier and the retinal pigment epithelium-further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion: Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.

KW - high resolution imaging

KW - magnetic resonance imaging

KW - MEMRI

KW - MRI microscopy

KW - RCS

KW - retinal degeneration

UR - http://www.scopus.com/inward/record.url?scp=81755183117&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81755183117&partnerID=8YFLogxK

U2 - 10.1002/jmri.22719

DO - 10.1002/jmri.22719

M3 - Article

C2 - 21964629

AN - SCOPUS:81755183117

VL - 34

SP - 1422

EP - 1429

JO - Journal of Magnetic Resonance Imaging

JF - Journal of Magnetic Resonance Imaging

SN - 1053-1807

IS - 6

ER -