Mammalian Srb/mediator complex is targeted by adenovirus E1A protein

Thomas G. Boyer; Michelle E.D. Martin; Emma Lees; Robert P. Ricciardi; Arnold J. Berk

doi:10.1038/20466

Mammalian Srb/mediator complex is targeted by adenovirus E1A protein

Thomas G. Boyer, Michelle E.D. Martin, Emma Lees, Robert P. Ricciardi, Arnold J. Berk

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

261 Scopus citations

Abstract

Adenovirus E1A proteins prepare the host cell for vital replication, stimulating cell cycling and vital transcription through interactions with critical cellular regulatory proteins such as RB and CBP. Here we show that the E1A zinc-finger domain that is required to activate transcription of vital early genes binds to a host-cell multiprotein complex containing homologues of yeast Srb/Mediator proteins. This occurs through a stable interaction with the human homologue of Caenorhabditis elegans SUR-2, a protein required for many developmental processes in the nematode. This human Srb/Mediator complex stimulates transcription in vitro in response to both the E1A zinc-finger and the herpes simplex virus VP16 activation domains. Interaction with human Sur-2 is also required for transcription to be activated by the activation domain of a transcription factor of the ETS- family in response to activated mitogen-activated protein (MAP) kinase.

Original language	English (US)
Pages (from-to)	276-279
Number of pages	4
Journal	Nature
Volume	399
Issue number	6733
DOIs	https://doi.org/10.1038/20466
State	Published - May 20 1999

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title = "Mammalian Srb/mediator complex is targeted by adenovirus E1A protein",

abstract = "Adenovirus E1A proteins prepare the host cell for vital replication, stimulating cell cycling and vital transcription through interactions with critical cellular regulatory proteins such as RB and CBP. Here we show that the E1A zinc-finger domain that is required to activate transcription of vital early genes binds to a host-cell multiprotein complex containing homologues of yeast Srb/Mediator proteins. This occurs through a stable interaction with the human homologue of Caenorhabditis elegans SUR-2, a protein required for many developmental processes in the nematode. This human Srb/Mediator complex stimulates transcription in vitro in response to both the E1A zinc-finger and the herpes simplex virus VP16 activation domains. Interaction with human Sur-2 is also required for transcription to be activated by the activation domain of a transcription factor of the ETS- family in response to activated mitogen-activated protein (MAP) kinase.",

author = "Boyer, {Thomas G.} and Martin, {Michelle E.D.} and Emma Lees and Ricciardi, {Robert P.} and Berk, {Arnold J.}",

note = "Funding Information: We acknowledge Dr. R. Chenraj Jain, President, Jain University Trust., Dr. N. Sundararajan, Vice Chancel-lor, Jain University and Prof. K. S. Shantamani, Chief Mentor, Jain University, Bangalore for their kind sup-port and encouragement and for providing facilities for carrying out this work. DBL thank Jain University for the constant support and encouragement for carry-ing out research aspects",

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doi = "10.1038/20466",

language = "English (US)",

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AU - Boyer, Thomas G.

AU - Martin, Michelle E.D.

AU - Lees, Emma

AU - Ricciardi, Robert P.

AU - Berk, Arnold J.

N1 - Funding Information: We acknowledge Dr. R. Chenraj Jain, President, Jain University Trust., Dr. N. Sundararajan, Vice Chancel-lor, Jain University and Prof. K. S. Shantamani, Chief Mentor, Jain University, Bangalore for their kind sup-port and encouragement and for providing facilities for carrying out this work. DBL thank Jain University for the constant support and encouragement for carry-ing out research aspects

PY - 1999/5/20

Y1 - 1999/5/20

N2 - Adenovirus E1A proteins prepare the host cell for vital replication, stimulating cell cycling and vital transcription through interactions with critical cellular regulatory proteins such as RB and CBP. Here we show that the E1A zinc-finger domain that is required to activate transcription of vital early genes binds to a host-cell multiprotein complex containing homologues of yeast Srb/Mediator proteins. This occurs through a stable interaction with the human homologue of Caenorhabditis elegans SUR-2, a protein required for many developmental processes in the nematode. This human Srb/Mediator complex stimulates transcription in vitro in response to both the E1A zinc-finger and the herpes simplex virus VP16 activation domains. Interaction with human Sur-2 is also required for transcription to be activated by the activation domain of a transcription factor of the ETS- family in response to activated mitogen-activated protein (MAP) kinase.

AB - Adenovirus E1A proteins prepare the host cell for vital replication, stimulating cell cycling and vital transcription through interactions with critical cellular regulatory proteins such as RB and CBP. Here we show that the E1A zinc-finger domain that is required to activate transcription of vital early genes binds to a host-cell multiprotein complex containing homologues of yeast Srb/Mediator proteins. This occurs through a stable interaction with the human homologue of Caenorhabditis elegans SUR-2, a protein required for many developmental processes in the nematode. This human Srb/Mediator complex stimulates transcription in vitro in response to both the E1A zinc-finger and the herpes simplex virus VP16 activation domains. Interaction with human Sur-2 is also required for transcription to be activated by the activation domain of a transcription factor of the ETS- family in response to activated mitogen-activated protein (MAP) kinase.

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Mammalian Srb/mediator complex is targeted by adenovirus E1A protein

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