MALDI tissue imaging: From biomarker discovery to clinical applications

Lisa H. Cazares, Dean A. Troyer, Binghe Wang, Richard R. Drake, O. John Semmes

Research output: Contribution to journalReview articlepeer-review

81 Scopus citations

Abstract

Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is a powerful tool for the generation of multidimensional spatial expression maps of biomolecules directly from a tissue section. From a clinical proteomics perspective, this method correlates molecular detail to histopathological changes found in patient-derived tissues, enhancing the ability to identify candidates for disease biomarkers. The unbiased analysis and spatial mapping of a variety of molecules directly from clinical tissue sections can be achieved through this method. Conversely, targeted IMS, by the incorporation of laser-reactive molecular tags onto antibodies, aptamers, and other affinity molecules, enables analysis of specific molecules or a class of molecules. In addition to exploring tissue during biomarker discovery, the integration of MALDI-IMS methods into existing clinical pathology laboratory practices could prove beneficial to diagnostics. Querying tissue for the expression of specific biomarkers in a biopsy is a critical component in clinical decision-making and such markers are a major goal of translational research. An important challenge in cancer diagnostics will be to assay multiple parameters in a single slide when tissue quantities are limited. The development of multiplexed assays that maximize the yield of information from a small biopsy will help meet a critical challenge to current biomarker research. This review focuses on the use of MALDI-IMS in biomarker discovery and its potential as a clinical diagnostic tool with specific reference to our application of this technology to prostate cancer.

Original languageEnglish (US)
Pages (from-to)17-27
Number of pages11
JournalAnalytical and Bioanalytical Chemistry
Volume401
Issue number1
DOIs
StatePublished - Jul 2011
Externally publishedYes

Keywords

  • Biomarker discovery
  • Cancer
  • Matrix-assisted laser desorption ionization tissue imaging

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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