Malabsorption of thiamine in folate deficient rats

L. Howard, C. Wagner, S. Schenker

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Sprague Dawley weanling rats were rendered folate deficient by feeding a folate free diet supplemented with succinylsulfathiazole for 3 or 10 mth. These rats exhibited a 10 and 5 fold reduction of liver and erythrocyte folate levels, respectively, at 3 mth and 110 fold reduction of folate in these organs after 10 mth, as compared with control animals. In the folate deficient rats there was a significant (30 to 50%) decrease in duodenal and jejunal absorption of low doses of thiamin hydrochloride (0.5 μM), whereas high doses of thiamin (17.5 μM), were assimilated normally. This differential absorption of these 2 doses of thiamin is explained in terms of a dual absorptive mechanism for thiamin, active and saturable at low concentrations and passive at higher levels of thiamin. 'Megaloblastic' changes were seen in the duodenum and jejunum, but not the ileum of folate deficient rats. Two wk of folate repletion restored the tissue folate levels essentially to normal and reversed the intestinal absorptive defect for thiamin, but the morphologic changes in the gut persisted.

Original languageEnglish (US)
Pages (from-to)1024-1032
Number of pages9
JournalJournal of Nutrition
Volume104
Issue number8
DOIs
StatePublished - 1974
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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