Major linear IgE epitopes of mountain cedar pollen allergen Jun a 1 map to the pectate lyase catalytic site

Terumi Midoro-Horiuti, Venkatarajan Mathura, Catherine H. Schein, Werner Braun, Shaoning Yu, Masanao Watanabe, J. Ching Lee, Edward G. Brooks, Randall M. Goldblum

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Resolution of the 3D structures and IgE epitopes of allergens may identify common or conserved features of allergens. Jun a 1, the predominant allergen in mountain cedar pollen, was chosen as a model for identifying common structural and functional features among a group of plant allergens. In this study, synthetic, overlapping peptides of Jun a 1 and sera from patients allergic to mountain cedar pollen were used to identify linear epitopes. A 3D model of Jun a 1 was produced using the Bacillus subtiles pectate lyase (PL) as a template and validated with biophysical measurements. This allowed mappings of four IgE binding sites on Jun a 1. Two of the epitopes mapped to turns or loops on the surface of the model structure. The other two epitopes mapped to the β-sheet region, homologous to the catalytic site of PL. This region of Jun a 1 is highly conserved in the group 1 allergens from other cedar trees as well as microbial PLs. The finding that two out of three major IgE epitopes map to highly conserved catalytic regions of group 1 cedar allergens may help to explain the high degree of cross-reactivity between cedar pollen allergens and might represent a pattern of reactivity common to other allergens with catalytic activity.

Original languageEnglish (US)
Pages (from-to)555-562
Number of pages8
JournalMolecular Immunology
Issue number8
StatePublished - Dec 2003
Externally publishedYes


  • Allergen structure
  • Cedar pollen hypersensitivity
  • Cry j 1
  • IgE epitope
  • Jun a 1
  • Juniperus ashei
  • Mountain cedar

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology


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