Maintenance and mobility of hemoglobin and water within the human erythrocyte after detergent disruption of the plasma membrane

Ivan L. Cameron, Laura A. Cox, Xi Rong Liu, Gary D. Fullerton

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Is an intact plasma membrane responsible for keeping hemoglobin and water within the human erythrocyte? If not, what is responsible? How free is Hb to move about within the erythrocyte? To answer these questions erythrocytes were taken for phase contrast microscopy, transmission electron microscopy (TEM), determination of water-holding capacity, and proton NMR studies both before and after membrane disruption with a nonionic detergent (Brij 58). Addition of 0.2% Brij to a D2O saline solution of hemoglobin (Hb) caused particles of Hb to appear and to aggregate. This aggregation of Hb caused the amplitude of the Hb proton NMR spectra to decrease. Thus, the less mobile the Hb the lower the Hb proton spectra amplitude. Erythrocytes washed in D2O saline showed proton NMR spectra of relatively low amplitude. Addition of Brij (0.2%) to these erythrocytes caused increased Hb mobility within these erythrocytes. The TEM of fixed and thin-sectioned erythrocytes treated with Brij showed disruption of the plasma membrane of all erythrocytes regardless of whether or not they had lost Hb. Brij-permeabilized erythrocytes washed in D2O saline or in a D2O K buffer maintained a higher heavy water-holding capacity upon centrifugation as compared to nonpermeabilized erythrocytes. The TEM of Brij-treated and washed erythrocyte "shells" revealed a continuous submembrane lamina but no other evidence of cytoskeletal elements. The water-holding capacity of the erythrocyte can be accounted for by the water-holding capacity of hemoglobin. The evidence favors a relatively immobile state of Hb and of water in the erythrocyte that is not immediately dependent on an intact plasma membrane but is attributed to interactions between Hb molecules and the submembrane lamina.

    Original languageEnglish (US)
    Pages (from-to)365-374
    Number of pages10
    JournalJournal of Cellular Physiology
    Volume149
    Issue number3
    StatePublished - Dec 1991

    Fingerprint

    Cell membranes
    Detergents
    Hemoglobins
    Erythrocytes
    Maintenance
    Cell Membrane
    Water
    Protons
    Transmission Electron Microscopy
    Nuclear magnetic resonance
    Transmission electron microscopy
    Cetomacrogol
    Deuterium Oxide
    Phase-Contrast Microscopy
    Centrifugation
    Sodium Chloride
    Buffers
    Agglomeration
    Membranes

    ASJC Scopus subject areas

    • Cell Biology
    • Clinical Biochemistry
    • Physiology

    Cite this

    Maintenance and mobility of hemoglobin and water within the human erythrocyte after detergent disruption of the plasma membrane. / Cameron, Ivan L.; Cox, Laura A.; Liu, Xi Rong; Fullerton, Gary D.

    In: Journal of Cellular Physiology, Vol. 149, No. 3, 12.1991, p. 365-374.

    Research output: Contribution to journalArticle

    Cameron, Ivan L. ; Cox, Laura A. ; Liu, Xi Rong ; Fullerton, Gary D. / Maintenance and mobility of hemoglobin and water within the human erythrocyte after detergent disruption of the plasma membrane. In: Journal of Cellular Physiology. 1991 ; Vol. 149, No. 3. pp. 365-374.
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