TY - JOUR
T1 - Maintenance and induction of cytochrome P-450 in cultured rat hepatocytes
AU - Engelmann, Gary L.
AU - Richardson, Arlan G.
AU - Fierer, Joshua A.
PY - 1985/5/1
Y1 - 1985/5/1
N2 - Maintenance of microsomal cytochrome P-450 content by cultured rat hepatocytes has proven an elusive goal. It is reported here that exogenous heme maintains cytochrome P-450 content of cultured rat hepatocytes at high levels during the first 72 h of incubation. The maintenance studies have been expanded to demonstrate the in vitro induction of cytochrome P-450 by phenobarbital treatment. The induction of P-450 in vitro by phenobarbital required the trace element, selenium, in the presence of exogenous heme. The present findings suggest that selenium, and other trace elements, may have an essential role in the formation of holocytochrome P-450 vitro.
AB - Maintenance of microsomal cytochrome P-450 content by cultured rat hepatocytes has proven an elusive goal. It is reported here that exogenous heme maintains cytochrome P-450 content of cultured rat hepatocytes at high levels during the first 72 h of incubation. The maintenance studies have been expanded to demonstrate the in vitro induction of cytochrome P-450 by phenobarbital treatment. The induction of P-450 in vitro by phenobarbital required the trace element, selenium, in the presence of exogenous heme. The present findings suggest that selenium, and other trace elements, may have an essential role in the formation of holocytochrome P-450 vitro.
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U2 - 10.1016/0003-9861(85)90175-4
DO - 10.1016/0003-9861(85)90175-4
M3 - Article
C2 - 3994378
AN - SCOPUS:0021879225
SN - 0003-9861
VL - 238
SP - 359
EP - 367
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -