Maintenance and culture of MSCs

Milos Marinkovic, David D Dean, Xiao Dong Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The phenotype and behavior of mesenchymal stem cells (MSCs) are regulated by cues from the extracellular environment. At present, the methods by which MSCs are isolated from tissue sources and commonly expanded ex vivo can decrease their number and render them functionally ineffective for transplantation and tissue regeneration. For this reason, MSCs used in both research and clinical applications should be maintained in culture conditions that replicate their native, tissue-specific microenvironments (niches) in vivo. Until recently, researchers have expended significant amounts of time and energy in developing a variety of models to untangle the relationship between MSC behavior and the unique combination of biochemical, architectural, and mechanical cues contained in the native stem cell niche. However, most of these approaches result in overly simplistic and artificial models that minimize the inherent complexity of the extracellular environment and provide information/data that are difficult to translate. In this chapter, we will describe a novel culture system, based on decellularized, stromal cell-derived extracellular matrix (ECM), which provides a physiologically relevant surrogate for the native stem cell niche and a sophisticated ex vivo environment for studying stem cell behavior, modeling age-related pathologies, and potentially controlling cell-fate for therapeutic applications.

Original languageEnglish (US)
Title of host publicationA Roadmap to Nonhematopoietic Stem Cell-Based Therapeutics
Subtitle of host publicationFrom the Bench to the Clinic
PublisherElsevier
Pages39-61
Number of pages23
ISBN (Electronic)9780128119204
ISBN (Print)9780128119211
DOIs
StatePublished - Jan 1 2018

Keywords

  • 3D tissue-specific ECM
  • Colony forming unit-fibroblast
  • Mesenchymal stem cell
  • Preservation of stem cell properties ex vivo
  • Stem cell niche
  • Tissue culture plastic

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Marinkovic, M., Dean, D. D., & Chen, X. D. (2018). Maintenance and culture of MSCs. In A Roadmap to Nonhematopoietic Stem Cell-Based Therapeutics: From the Bench to the Clinic (pp. 39-61). Elsevier. https://doi.org/10.1016/B978-0-12-811920-4.00003-3