Magnocellular vasopressinergic neurons in explant cultures are rescued from cell death by ciliary neurotrophic factor and leukemia inhibiting factor

L. Vutskits, Viktor Bartanusz, M. F. Schulz, J. Z. Kiss

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Selective death of magnocellular vasopressinergic neurons in the hypothalamus has been reported in cases of hereditary and idiopathic diabetes insipidus and after experimental lesions of the hypothalamo-neurohypophyseal pathway. To identify trophic factors that promote survival of these neurons, an in vitro model system was established in which organotypic cultures of the rat hypothalamic paraventricular nucleus were maintained in chemically- defined medium. We observe that the majority of magnocellular vasopressinergic neurons die in these cultures, while other cell populations such as corticotrophin-releasing factor producing parvicellular and oxytocin producing magnocellular cells retain a well preserved cytoarchitectonic organization. Degenerating vasopressinergic cells exhibit morphological signs of apoptosis and stained positively when analysed by the terminal deoxynucleotidyl transferase biotinylated dUTP nick end-labelling assay. Partial survival of vasopressinergic neurons occurred after co-culturing the paraventricular nucleus with neurohypophyseal explants, indicating that target-derived factors may be required for the survival of these neurons. Cell survival is dramatically increased by the administration of ciliary neurotrophic factor and leukemia inhibiting factor, but not by interleukin 6 or the members of the neurotrophin family. Reverse transcription-polymerase chain reaction followed by Southern analysis shows the presence of ciliary neurotrophic factor messenger RNA in the neurohypophysis. Thus, endogenous ciliary neurotrophic factor and leukemia inhibiting factor, produced by neurohypophyseal cells may function as a physiological survival factor for neurosecretory vasopressinergic neurons.

Original languageEnglish (US)
Pages (from-to)571-582
Number of pages12
JournalNeuroscience
Volume87
Issue number3
DOIs
StatePublished - Aug 4 1998
Externally publishedYes

Fingerprint

Ciliary Neurotrophic Factor
Leukemia
Cell Death
Neurons
Paraventricular Hypothalamic Nucleus
Posterior Pituitary Gland
Diabetes Insipidus
DNA Nucleotidylexotransferase
Corticotropin-Releasing Hormone
Nerve Growth Factors
Oxytocin
Hypothalamus
Reverse Transcription
Interleukin-6
Cell Survival
Organizations
Apoptosis
Polymerase Chain Reaction
Messenger RNA
Population

Keywords

  • Apoptosis
  • CNTF
  • LIF
  • Organotypic culture
  • Paraventricular nucleus
  • Vasopressin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Magnocellular vasopressinergic neurons in explant cultures are rescued from cell death by ciliary neurotrophic factor and leukemia inhibiting factor. / Vutskits, L.; Bartanusz, Viktor; Schulz, M. F.; Kiss, J. Z.

In: Neuroscience, Vol. 87, No. 3, 04.08.1998, p. 571-582.

Research output: Contribution to journalArticle

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