Magnetic resonance imaging of tissue and vascular layers in the cat retina

Qiang Shen, Haiying Cheng, Machelle T. Pardue, Thomas F. Chang, Govind Nair, Van Toi Vo, Ross D. Shonat, Timothy Q. Duong

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Purpose: To report the visual resolution of multiple cell and vascular "layers" in the cat retina using MRI. Materials and Methods: T 2- and diffusion-weighted MRI at 4.7 Tesla was performed. Layer-specific thickness, T2, spin density, apparent diffusion coefficient perpendicular (ADC) and parallel (ADC ) to the retinal surface were tabulated. T1-weighted MRI was acquired before and after Intravenous administration of Gd-DTPA and subtraction images were obtained. Histology was performed for validation. Results: Three distinct "layers" were observed. The inner strip nearest to the vitreous (exhibiting large T2, ADC, spin density with Gd-DTPA enhancement) overlapped the ganglion cell layer, bipolar cell layer, and the embedded retinal vascular layer. The middle strip (exhibiting small T 2, ADC, spin density without Gd-DTPA enhancement) overlapped the photoreceptor cell layer and the inner and outer segments. The outer strip (exhibiting large T2, ADC, spin density with Gd-DTPA enhancement) overlapped the tapetum and choroidal vascular layer. T2, spin density, ADC and ADC of different "layers" were tabulated. The inner strip was slightly thicker than the other two strips. The total thickness, including neural and nonneural retina, was 358 ± 13μm (N = 6) by MRI and 319 ± 77 μm (N = 5) by histology. Conclusion: MRI provides a noninvasive tool to study the retina with laminar specificity without depth limitation.

Original languageEnglish (US)
Pages (from-to)465-472
Number of pages8
JournalJournal of Magnetic Resonance Imaging
Volume23
Issue number4
DOIs
StatePublished - Apr 2006

Keywords

  • Cortical columns
  • Cortical layers
  • Gd-DTPA
  • High-resolution MRI
  • Laminar specificity

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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