Magnetic Field-Activated Sensing of mRNA in Living Cells

Saira F. Bakshi; Nataliia Guz; Andrey Zakharchenko; Han Deng; Alexei V. Tumanov; Craig D. Woodworth; Sergiy Minko; Dmitry M. Kolpashchikov; Evgeny Katz

doi:10.1021/jacs.7b06022

Magnetic Field-Activated Sensing of mRNA in Living Cells

Saira F. Bakshi, Nataliia Guz, Andrey Zakharchenko, Han Deng, Alexei V. Tumanov, Craig D. Woodworth, Sergiy Minko, Dmitry M. Kolpashchikov, Evgeny Katz

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Detection of specific mRNA in living cells has attracted significant attention in the past decade. Probes that can be easily delivered into cells and activated at the desired time can contribute to understanding translation, trafficking and degradation of mRNA. Here we report a new strategy termed magnetic field-activated binary deoxyribozyme (MaBiDZ) sensor that enables both efficient delivery and temporal control of mRNA sensing by magnetic field. MaBiDZ uses two species of magnetic beads conjugated with different components of a multicomponent deoxyribozyme (DZ) sensor. The DZ sensor is activated only in the presence of a specific target mRNA and when a magnetic field is applied. Here we demonstrate that MaBiDZ sensor can be internalized in live MCF-7 breast cancer cells and activated by a magnetic field to fluorescently report the presence of specific mRNA, which are cancer biomarkers.

Original language	English (US)
Pages (from-to)	12117-12120
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	139
Issue number	35
DOIs	https://doi.org/10.1021/jacs.7b06022
State	Published - Sep 6 2017

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/jacs.7b06022

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abstract = "Detection of specific mRNA in living cells has attracted significant attention in the past decade. Probes that can be easily delivered into cells and activated at the desired time can contribute to understanding translation, trafficking and degradation of mRNA. Here we report a new strategy termed magnetic field-activated binary deoxyribozyme (MaBiDZ) sensor that enables both efficient delivery and temporal control of mRNA sensing by magnetic field. MaBiDZ uses two species of magnetic beads conjugated with different components of a multicomponent deoxyribozyme (DZ) sensor. The DZ sensor is activated only in the presence of a specific target mRNA and when a magnetic field is applied. Here we demonstrate that MaBiDZ sensor can be internalized in live MCF-7 breast cancer cells and activated by a magnetic field to fluorescently report the presence of specific mRNA, which are cancer biomarkers.",

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AU - Bakshi, Saira F.

AU - Guz, Nataliia

AU - Zakharchenko, Andrey

AU - Deng, Han

AU - Tumanov, Alexei V.

AU - Woodworth, Craig D.

AU - Minko, Sergiy

AU - Kolpashchikov, Dmitry M.

AU - Katz, Evgeny

PY - 2017/9/6

Y1 - 2017/9/6

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AB - Detection of specific mRNA in living cells has attracted significant attention in the past decade. Probes that can be easily delivered into cells and activated at the desired time can contribute to understanding translation, trafficking and degradation of mRNA. Here we report a new strategy termed magnetic field-activated binary deoxyribozyme (MaBiDZ) sensor that enables both efficient delivery and temporal control of mRNA sensing by magnetic field. MaBiDZ uses two species of magnetic beads conjugated with different components of a multicomponent deoxyribozyme (DZ) sensor. The DZ sensor is activated only in the presence of a specific target mRNA and when a magnetic field is applied. Here we demonstrate that MaBiDZ sensor can be internalized in live MCF-7 breast cancer cells and activated by a magnetic field to fluorescently report the presence of specific mRNA, which are cancer biomarkers.

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Magnetic Field-Activated Sensing of mRNA in Living Cells

Abstract

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