Macrophage CGI-58 deficiency activates ros-inflammasome pathway to promote insulin resistance in mice

Hongming Miao, Juanjuan Ou, Yinyan Ma, Feng Guo, Zhenggang Yang, Melvin Wiggins, Chaohong Liu, Wenxia Song, Xianlin Han, Miao Wang, Qiang Cao, Bik Ho Florence Chung, Dan Yang, Houjie Liang, Bingzhong Xue, Hang Shi, Lixia Gan, Liqing Yu

Research output: Contribution to journalArticlepeer-review

77 Scopus citations


Overnutrition activates a proinflammatory program in macrophages to induce insulin resistance (IR), but its molecular mechanisms remain incompletely understood. Here, we show that saturated fatty acid and lipopolysaccharide, two factors implicated in high-fat diet (HFD)-induced IR, suppress macrophage CGI-58 expression. Macrophage-specific CGI-58 knockout (MaKO) in mice aggravates HFD-induced glucose intolerance and IR, which is associated with augmented systemic/tissue inflammation and proinflammatory activation of adipose tissue macrophages. CGI-58-deficient macrophages exhibit mitochondrial dysfunction due to defective peroxisome proliferator-activated receptor (PPAR)γ signaling. Consequently, they overproduce reactive oxygen species (ROS) to potentiate secretion of proinflammatory cytokines by activating NLRP3 inflammasome. Anti-ROS treatment or NLRP3 silencing prevents CGI-58-deficient macrophages from oversecreting proinflammatory cytokines and from inducing proinflammatory signaling and IR in the cocultured fat slices. Anti-ROS treatment also prevents exacerbation of inflammation and IR in HFD-fed MaKO mice. Our data thus establish CGI-58 as a suppressor of overnutrition-induced NLRP3 inflammasome activation in macrophages.

Original languageEnglish (US)
Pages (from-to)223-235
Number of pages13
JournalCell Reports
Issue number1
StatePublished - Oct 4 2014
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


Dive into the research topics of 'Macrophage CGI-58 deficiency activates ros-inflammasome pathway to promote insulin resistance in mice'. Together they form a unique fingerprint.

Cite this