Lytic replication-defective Kaposi's sarcoma-associated herpesvirus: Potential role in infection and malignant transformation

Jian Hong Deng, Yan Jin Zhang, Xin Ping Wang, Shou Jiang Gao

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Defective viruses often have pivotal roles in virus-induced diseases. Although Kaposi's sarcoma-associated herpesvirus (KSHV) is etiologically associated with Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL), defective KSHV has not been reported. Using differential genetic screening methods, we show that defective KSHV is present in KS tumors and PEL cell lines. To investigate the role of defective viruses in KSHV-induced pathogenesis, we isolated and characterized a lytic replication-defective KSHV, KV-1, containing an 82-kb genomic deletion of solely lytic genes. Cells harboring KV-1 escaped G0/G1 apoptosis induced by spontaneous lytic replication occurred in cells infected with regular KSHV but maintained efficient latent replication. Consequently, KV-1-infected cells had phenotypes of enhanced cell proliferation and transformation potentials. Importantly, KV-1 was packaged as infectious virions by using regular KSHV as helpers, and KV-1-like variants were detected in cultures of two of five KSHV cell lines and 1 of 18 KS tumors. These results point to a potential role for defective viruses in the regulation of KSHV infection and malignant transformation.

Original languageEnglish (US)
Pages (from-to)11108-11120
Number of pages13
JournalJournal of virology
Volume78
Issue number20
DOIs
StatePublished - Oct 2004

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Lytic replication-defective Kaposi's sarcoma-associated herpesvirus: Potential role in infection and malignant transformation'. Together they form a unique fingerprint.

  • Cite this