Lymphocyte phenotypes in infants are altered by separation of blood on density gradients

S. G. Kutvirt, S. L. Lewis, T. L. Simon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Flow cytometry techniques for immunophenotyping have revolutionised the diagnosis and monitoring of paediatric immunological disorders. Although recent studies in adult subjects discourage the use of density gradients for cell preparation prior to phenotyping, these procedures continue to be used. The purpose of this study was to determine the effect of density gradient separation on lymphocyte phenotypes from neonates, infants, and adults as compared to whole blood determinations. Subset distributions were different with the two procedures. In all three groups, CD19+ (B cell) and CD8+ (suppressor/cytotoxic T cell) percentages were significantly lower and CD3- CD56+ (NK cell) percentages were significantly higher in the density gradient separated cells. The loss of CD8+ cells in density gradient separation was shown to be a selective event. The CD8+Cd11b- (cytotoxic T) subset percentages were lower in the density gradient separated cells, while the percentages of CD8+CD11b+ (suppressor T) cells were not affected by separation procedure. Because of the selective loss of lymphocytes on density gradients, the use of a whole blood technique for immunophenotyping in paediatric subjects is recommended.

Original languageEnglish (US)
Pages (from-to)321-328
Number of pages8
JournalBritish Journal of Biomedical Science
Volume50
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Lymphocytes
Immunophenotyping
Pediatrics
Blood
T-cells
Phenotype
Cell Count
T-Lymphocytes
Immunologic Monitoring
Flow cytometry
Natural Killer Cells
Flow Cytometry
B-Lymphocytes
Cells
Newborn Infant
Monitoring

Keywords

  • Centrifugation, density gradient
  • Flow cytometry
  • Hemolysis
  • Immunophenotyping

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lymphocyte phenotypes in infants are altered by separation of blood on density gradients. / Kutvirt, S. G.; Lewis, S. L.; Simon, T. L.

In: British Journal of Biomedical Science, Vol. 50, No. 4, 1993, p. 321-328.

Research output: Contribution to journalArticle

Kutvirt, S. G. ; Lewis, S. L. ; Simon, T. L. / Lymphocyte phenotypes in infants are altered by separation of blood on density gradients. In: British Journal of Biomedical Science. 1993 ; Vol. 50, No. 4. pp. 321-328.
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