TY - JOUR
T1 - Lymphocyte accumulation in the spleen of retinoic acid receptor-related orphan receptor γ-deficient mice
AU - Zhang, Nu
AU - Guo, Jian
AU - He, You Wen
PY - 2003/8/15
Y1 - 2003/8/15
N2 - The hormone nuclear receptor retinoic acid receptor-related orphan receptor γ (RORγ) plays important roles in thymocyte development and lymphoid organogenesis. RORγ and its thymus-specific isoform RORγt are expressed in the thymus, but not in the spleen and bone marrow (BM). However, RORγ-/- mice have 2- to 3-fold more splenocytes than wild-type controls due to an accumulation of conventional resting B lymphocytes. The increase in B lymphocytes in RORγ-/- mice is caused neither by abnormal B cell development in the BM nor by an obvious defect in the peripheral T cell compartment. Furthermore, analyses of BM chimeras using either RORγ-/- or recombinase-activating gene-2-/- mice as recipients and wild-type or RORγ -/- mice as donors, respectively, demonstrate that the splenic microenvironment of RORγ-/- mice is defective, since wild-type T and B lymphocytes accumulated in these chimeric mice. In addition, T lymphocyte homeostasis was altered due to a lowered thymic output in RORγ-/- mice. Collectively, these results suggest that RORγ regulates lymphocyte homeostasis at multiple levels.
AB - The hormone nuclear receptor retinoic acid receptor-related orphan receptor γ (RORγ) plays important roles in thymocyte development and lymphoid organogenesis. RORγ and its thymus-specific isoform RORγt are expressed in the thymus, but not in the spleen and bone marrow (BM). However, RORγ-/- mice have 2- to 3-fold more splenocytes than wild-type controls due to an accumulation of conventional resting B lymphocytes. The increase in B lymphocytes in RORγ-/- mice is caused neither by abnormal B cell development in the BM nor by an obvious defect in the peripheral T cell compartment. Furthermore, analyses of BM chimeras using either RORγ-/- or recombinase-activating gene-2-/- mice as recipients and wild-type or RORγ -/- mice as donors, respectively, demonstrate that the splenic microenvironment of RORγ-/- mice is defective, since wild-type T and B lymphocytes accumulated in these chimeric mice. In addition, T lymphocyte homeostasis was altered due to a lowered thymic output in RORγ-/- mice. Collectively, these results suggest that RORγ regulates lymphocyte homeostasis at multiple levels.
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U2 - 10.4049/jimmunol.171.4.1667
DO - 10.4049/jimmunol.171.4.1667
M3 - Article
C2 - 12902464
AN - SCOPUS:0041589523
SN - 0022-1767
VL - 171
SP - 1667
EP - 1675
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -