@article{d03403a8530a46d888905645533da504,
title = "Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche",
abstract = "In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages. Mondor et al. demonstrate that subcapsular sinus macrophages (SSMs) self-maintain by proliferation in the adult mouse lymph node and that homeostasis of SSMs and medullary sinus macrophages (MSMs) relies on secretion of CSF-1 by neighboring lymphatic endothelial cells (LECs).",
keywords = "colony stimulating factor-1, homeostasis, lymph node, macrophages, niche, ontogeny, stromal cells",
author = "Isabelle Mondor and Myriam Baratin and Marine Lagueyrie and Lisa Saro and Sandrine Henri and Rebecca Gentek and Delphine Suerinck and Wolfgang Kastenmuller and Jiang, {Jean X.} and Marc Baj{\'e}noff",
note = "Funding Information: We thank Taija Makinen (Uppsala University, Department Immunology, Genetics and Pathology, Sweden) for providing the Prox1 CreERT2 mice, Sahil Adriouch (Normandie University, Institute for Research and Innovation in Biomedicine, France) for providing VSV, and the CIML core facilities for animal housing and flow cytometry. This study was funded by grants from the NIH ( AG045040 ), Agence National pour la Recherche ( ANR-10-INBS-04-01 France Bio Imaging and ANR-17-CE15-0015-01 STROMAC ) and from the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation program grant agreement N° 647257- STROMA . This work was supported by institutional grants from INSERM , CNRS and Aix-Marseille University to the CIML. Funding Information: We thank Taija Makinen (Uppsala University, Department Immunology, Genetics and Pathology, Sweden) for providing the Prox1CreERT2 mice, Sahil Adriouch (Normandie University, Institute for Research and Innovation in Biomedicine, France) for providing VSV, and the CIML core facilities for animal housing and flow cytometry. This study was funded by grants from the NIH (AG045040), Agence National pour la Recherche (ANR-10-INBS-04-01 France Bio Imaging and ANR-17-CE15-0015-01 STROMAC) and from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program grant agreement N° 647257- STROMA. This work was supported by institutional grants from INSERM, CNRS and Aix-Marseille University to the CIML. Conceptualization, I.M. M. Baratin, and M. Baj{\'e}noff; Methodology, I.M. M. Baratin, and M. Baj{\'e}noff; Investigation, I.M. M. Baratin, L.S. M.L. S.H. D.S. R.G. and M. Baj{\'e}noff; Resources, S.H. W.K. J.X.J.; Writing – Original Draft, I.M. M. Baratin and M. Baj{\'e}noff; Writing – Review & Editing, I.M. M. Baratin, R.G. and M. Baj{\'e}noff; Supervision, I.M. M. Baratin, and M. Baj{\'e}noff; Project administration, M. Baj{\'e}noff; Funding Acquisition, M. Baj{\'e}noff. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jun,
day = "18",
doi = "10.1016/j.immuni.2019.04.002",
language = "English (US)",
volume = "50",
pages = "1453--1466.e4",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "6",
}