Abstract
In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages. Mondor et al. demonstrate that subcapsular sinus macrophages (SSMs) self-maintain by proliferation in the adult mouse lymph node and that homeostasis of SSMs and medullary sinus macrophages (MSMs) relies on secretion of CSF-1 by neighboring lymphatic endothelial cells (LECs).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1453-1466.e4 |
| Journal | Immunity |
| Volume | 50 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 18 2019 |
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Keywords
- colony stimulating factor-1
- homeostasis
- lymph node
- macrophages
- niche
- ontogeny
- stromal cells
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases
Cite this
Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche. / Mondor, Isabelle; Baratin, Myriam; Lagueyrie, Marine; Saro, Lisa; Henri, Sandrine; Gentek, Rebecca; Suerinck, Delphine; Kastenmuller, Wolfgang; Jiang, Jean X; Bajénoff, Marc.
In: Immunity, Vol. 50, No. 6, 18.06.2019, p. 1453-1466.e4.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche
AU - Mondor, Isabelle
AU - Baratin, Myriam
AU - Lagueyrie, Marine
AU - Saro, Lisa
AU - Henri, Sandrine
AU - Gentek, Rebecca
AU - Suerinck, Delphine
AU - Kastenmuller, Wolfgang
AU - Jiang, Jean X
AU - Bajénoff, Marc
PY - 2019/6/18
Y1 - 2019/6/18
N2 - In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages. Mondor et al. demonstrate that subcapsular sinus macrophages (SSMs) self-maintain by proliferation in the adult mouse lymph node and that homeostasis of SSMs and medullary sinus macrophages (MSMs) relies on secretion of CSF-1 by neighboring lymphatic endothelial cells (LECs).
AB - In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages. Mondor et al. demonstrate that subcapsular sinus macrophages (SSMs) self-maintain by proliferation in the adult mouse lymph node and that homeostasis of SSMs and medullary sinus macrophages (MSMs) relies on secretion of CSF-1 by neighboring lymphatic endothelial cells (LECs).
KW - colony stimulating factor-1
KW - homeostasis
KW - lymph node
KW - macrophages
KW - niche
KW - ontogeny
KW - stromal cells
UR - http://www.scopus.com/inward/record.url?scp=85066316390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066316390&partnerID=8YFLogxK
U2 - 10.1016/j.immuni.2019.04.002
DO - 10.1016/j.immuni.2019.04.002
M3 - Article
VL - 50
SP - 1453-1466.e4
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 6
ER -