Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche

Isabelle Mondor, Myriam Baratin, Marine Lagueyrie, Lisa Saro, Sandrine Henri, Rebecca Gentek, Delphine Suerinck, Wolfgang Kastenmuller, Jean X Jiang, Marc Bajénoff

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

In lymph nodes, subcapsular sinus macrophages (SSMs) form an immunological barrier that monitors lymph drained from peripheral tissues. Upon infection, SSMs activate B and natural killer T (NKT) cells while secreting inflammatory mediators. Here, we investigated the mechanisms regulating development and homeostasis of SSMs. Embryonic SSMs originated from yolk sac hematopoiesis and were replaced by a postnatal wave of bone marrow (BM)-derived monocytes that proliferated to establish the adult SSM network. The SSM network self-maintained by proliferation with minimal BM contribution. Upon pathogen-induced transient deletion, BM-derived cells contributed to restoring the SSM network. Lymphatic endothelial cells (LECs) were the main source of CSF-1 within the lymph node and conditional deletion of Csf1 in adult LECs decreased the network of SSMs and medullary sinus macrophages (MSMs). Thus, SSMs have a dual hematopoietic origin, and LECs are essential to the niche supporting these macrophages. Mondor et al. demonstrate that subcapsular sinus macrophages (SSMs) self-maintain by proliferation in the adult mouse lymph node and that homeostasis of SSMs and medullary sinus macrophages (MSMs) relies on secretion of CSF-1 by neighboring lymphatic endothelial cells (LECs).

Original languageEnglish (US)
Pages (from-to)1453-1466.e4
JournalImmunity
Volume50
Issue number6
DOIs
StatePublished - Jun 18 2019

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Keywords

  • colony stimulating factor-1
  • homeostasis
  • lymph node
  • macrophages
  • niche
  • ontogeny
  • stromal cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Mondor, I., Baratin, M., Lagueyrie, M., Saro, L., Henri, S., Gentek, R., Suerinck, D., Kastenmuller, W., Jiang, J. X., & Bajénoff, M. (2019). Lymphatic Endothelial Cells Are Essential Components of the Subcapsular Sinus Macrophage Niche. Immunity, 50(6), 1453-1466.e4. https://doi.org/10.1016/j.immuni.2019.04.002