Luminescent biocompatible quantum dots a tool for immunosorbent assay design

Ellen R. Goldman, H. Tetsuo Uyeda, Andrew Hayhurst, Hedi Mattoussi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

We have developed several conjugation strategies based on noncovalent self-assembly for the attachment of proteins and other biomolecules to water-soluble luminescent colloidal semiconductor nanocrystals (quantum dots [QDs]). The resulting QD-protein conjugates were employed in designing a variety of bioinspired applications, including single and multiplexed immunosorbent assays to detect toxins and small molecule explosives. In these studies we showed that QD fluorophores offer several important advantages. In particular, their tunable broad excitation, spectra combined with narrow fluorescence emission peaks permit single-line excitation of multiple color nanocrystals, with facile signal deconvolution to extract individual contributions from each population (e.g., size) of QDs in. multiplexed assays. Furthermore, the QDs strong resistance to photobleaching under continuous illumination relative to many organic dyes makes them ideal fluorophores for long-term cellular imaging studies. This chapter details the materials and methods for the synthesis of surface-functionalized CdSe-ZnS core-shell QDs, the construction and preparation of recombinant proteins, the conjugation of antibodies (and antibody fragments) to QDs, and the use of antibody-conjugated QDs in fluoroimmunoassays.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages207-227
Number of pages21
Volume374
DOIs
StatePublished - Feb 2 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume374
ISSN (Print)10643745

Fingerprint

Quantum Dots
Immunosorbents
Fluoroimmunoassay
Photobleaching
Immunoglobulin Fragments
Antibodies
Population Density
Lighting
Recombinant Proteins
Nanoparticles
Proteins
Coloring Agents
Color
Fluorescence
Water

Keywords

  • Competition assay
  • Fluoroimmunoassay
  • QD fluorophores
  • QD-antibody conjugates

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Goldman, E. R., Uyeda, H. T., Hayhurst, A., & Mattoussi, H. (2007). Luminescent biocompatible quantum dots a tool for immunosorbent assay design. In Methods in Molecular Biology (Vol. 374, pp. 207-227). (Methods in Molecular Biology; Vol. 374). https://doi.org/10.1385/1-59745-369-2:207

Luminescent biocompatible quantum dots a tool for immunosorbent assay design. / Goldman, Ellen R.; Uyeda, H. Tetsuo; Hayhurst, Andrew; Mattoussi, Hedi.

Methods in Molecular Biology. Vol. 374 2007. p. 207-227 (Methods in Molecular Biology; Vol. 374).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Goldman, ER, Uyeda, HT, Hayhurst, A & Mattoussi, H 2007, Luminescent biocompatible quantum dots a tool for immunosorbent assay design. in Methods in Molecular Biology. vol. 374, Methods in Molecular Biology, vol. 374, pp. 207-227. https://doi.org/10.1385/1-59745-369-2:207
Goldman ER, Uyeda HT, Hayhurst A, Mattoussi H. Luminescent biocompatible quantum dots a tool for immunosorbent assay design. In Methods in Molecular Biology. Vol. 374. 2007. p. 207-227. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-369-2:207
Goldman, Ellen R. ; Uyeda, H. Tetsuo ; Hayhurst, Andrew ; Mattoussi, Hedi. / Luminescent biocompatible quantum dots a tool for immunosorbent assay design. Methods in Molecular Biology. Vol. 374 2007. pp. 207-227 (Methods in Molecular Biology).
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