Low lactate dehydrogenase and high mitochondrial glycerol phosphate dehydrogenase in pancreatic β-cells. Potential role in nutrient sensing

N. Sekine, V. Cirulli, R. Regazzi, L. J. Brown, E. Gine, J. Tamarit-Rodriguez, M. Girotti, S. Marie, M. J. MacDonald, C. B. Wollheim, G. A. Rutter

Research output: Contribution to journalArticlepeer-review

332 Scopus citations

Abstract

Nutrient metabolism was examined with regard to insulin secretion in purified rat islet β- and non-β-cells, β-cell lines, and hepatocytes. Lactate dehydrogenase (LDH) activity (nanomoles · min-1 · mg protein- 1) was remarkably low in the glucose-sensitive INS-1 cell line (15.7) and in β-cells (22.3). Thus, β-cell LDH was respectively 8-, 122-, 17-, and 136- fold lower than in islet non-β-, liver, HIT-T15, and RINm5F cells. Plasma membrane lactate transport activity was 3-10-fold lower in β- or INS-1 cells than in the other cell types. Conversely, mitochondrial glycerol phosphate dehydrogenase was strongly expressed only in β- and INS-1 cells. The significance of these findings to nutrient recognition was explored using INS-1 cells as a model of native β-cells. Glucose-stimulated lactate output and glucose utilization were, respectively, 12- and 5-fold lower in INS-1 than in RINm5F cells. Each process was entirely blocked by respiratory chain inhibitors in INS-1 cells, whereas glucose utilization was barely affected and lactate output stimulated in RINm5F cells. Glucose oxidation represented 73% of total utilization in INS-1 cells, but only 9% in RINm5F cells. Absolute rates of glucose oxidation, and the extent of mitochondrial NAD(P) reduction, were similar in the two cell types, and glucose stimulated insulin secretion 1.9-fold in INS-1 and 1.4-fold in RINm5F cells. The mitochondrial substrates, monomethyl succinate, pyruvate, and leucine, each triggered secretion in INS-1 cells. The balance of LDH, plasma membrane lactate transport, and mitochondrial glycerol phosphate dehydrogenase activities therefore appear to be important in β- and INS-1 cell glucose recognition to ensure that mitochondrial oxidation is the principle fate of pyruvate and NADH produced by glycolysis. The resultant close coupling of glycolysis with mitochondrial oxidation explains the absence in β-cells of Crabtree and Pasteur effects.

Original languageEnglish (US)
Pages (from-to)4895-4902
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number7
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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