Abstract
Lovastatin, an inhibitor of protein prenylation, was reported to inhibit DNA synthesis and induce apoptosis in cultured cells. This report describes the morphological consequences of lovastatin treatment. Lovastatin (50 μM) induced mesangial cell rounding and disassembly of actin stress fibers within 24 to 48 h. After 48 to 72 h of lovastatin treatment, the cells detached from the substratum and underwent apoptotic cell death as evidenced by condensed nuclear chromatin, nuclear fragmentation, cell blebbing and decrease in cell size. Time lapse cinematography revealed that lovastatin caused cell rounding by either inhibiting cytokinesis or cell spreading following cytokinesis. Lovastatin-induced cell rounding, detachment, and apoptosis were dependent upon cell proliferation. These effects were prevented by serum deprivation to inhibit cell proliferation or by plating cells at densities which resulted in contact inhibition of cell growth. Lovastatin-induced mesangial cell rounding and apoptosis were also prevented by the inclusion of the isoprenoids all-trans-farnesol or all-trans-geranylgeraniol in the incubation medium. These results indicate that the effects of lovastatin were mediated by inhibition of protein isoprenylation because exogenous all-trans-geranylgeraniol can be used only in protein prenylation. The small GTP-binding protein RhoA, which may be important for cell spreading and cytokinesis, accumulated in the cytosol following treatment with lovastatin, suggestive of its inactivation. This effect was also prevented by the inclusion of either farnesol or geranylgeraniol in the incubation medium. Thus, lovastatin-induced apoptosis in mesangial cells occurs by interfering with prenylation dependent mitotic and post-mitotic events.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 13-24 |
| Number of pages | 12 |
| Journal | Biochimica et Biophysica Acta - Molecular Cell Research |
| Volume | 1359 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 30 1997 |
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Keywords
- Cytokinesis
- Farnesol
- Geranylgeraniol
- Mesangial cell
- Protein prenylation
- RhoA
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Biophysics
Cite this
Lovastatin induces apoptosis by inhibiting mitotic and post-mitotic events in cultured mesangial cells. / Ghosh, Paramita M.; Mott, Glen E.; Ghosh-choudhury, Nandini; Radnik, Robert A.; Stapleton, Marissa L.; Ghidoni, John J.; Kreisberg, Jeffrey I.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1359, No. 1, 30.10.1997, p. 13-24.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lovastatin induces apoptosis by inhibiting mitotic and post-mitotic events in cultured mesangial cells
AU - Ghosh, Paramita M.
AU - Mott, Glen E.
AU - Ghosh-choudhury, Nandini
AU - Radnik, Robert A.
AU - Stapleton, Marissa L.
AU - Ghidoni, John J.
AU - Kreisberg, Jeffrey I.
PY - 1997/10/30
Y1 - 1997/10/30
N2 - Lovastatin, an inhibitor of protein prenylation, was reported to inhibit DNA synthesis and induce apoptosis in cultured cells. This report describes the morphological consequences of lovastatin treatment. Lovastatin (50 μM) induced mesangial cell rounding and disassembly of actin stress fibers within 24 to 48 h. After 48 to 72 h of lovastatin treatment, the cells detached from the substratum and underwent apoptotic cell death as evidenced by condensed nuclear chromatin, nuclear fragmentation, cell blebbing and decrease in cell size. Time lapse cinematography revealed that lovastatin caused cell rounding by either inhibiting cytokinesis or cell spreading following cytokinesis. Lovastatin-induced cell rounding, detachment, and apoptosis were dependent upon cell proliferation. These effects were prevented by serum deprivation to inhibit cell proliferation or by plating cells at densities which resulted in contact inhibition of cell growth. Lovastatin-induced mesangial cell rounding and apoptosis were also prevented by the inclusion of the isoprenoids all-trans-farnesol or all-trans-geranylgeraniol in the incubation medium. These results indicate that the effects of lovastatin were mediated by inhibition of protein isoprenylation because exogenous all-trans-geranylgeraniol can be used only in protein prenylation. The small GTP-binding protein RhoA, which may be important for cell spreading and cytokinesis, accumulated in the cytosol following treatment with lovastatin, suggestive of its inactivation. This effect was also prevented by the inclusion of either farnesol or geranylgeraniol in the incubation medium. Thus, lovastatin-induced apoptosis in mesangial cells occurs by interfering with prenylation dependent mitotic and post-mitotic events.
AB - Lovastatin, an inhibitor of protein prenylation, was reported to inhibit DNA synthesis and induce apoptosis in cultured cells. This report describes the morphological consequences of lovastatin treatment. Lovastatin (50 μM) induced mesangial cell rounding and disassembly of actin stress fibers within 24 to 48 h. After 48 to 72 h of lovastatin treatment, the cells detached from the substratum and underwent apoptotic cell death as evidenced by condensed nuclear chromatin, nuclear fragmentation, cell blebbing and decrease in cell size. Time lapse cinematography revealed that lovastatin caused cell rounding by either inhibiting cytokinesis or cell spreading following cytokinesis. Lovastatin-induced cell rounding, detachment, and apoptosis were dependent upon cell proliferation. These effects were prevented by serum deprivation to inhibit cell proliferation or by plating cells at densities which resulted in contact inhibition of cell growth. Lovastatin-induced mesangial cell rounding and apoptosis were also prevented by the inclusion of the isoprenoids all-trans-farnesol or all-trans-geranylgeraniol in the incubation medium. These results indicate that the effects of lovastatin were mediated by inhibition of protein isoprenylation because exogenous all-trans-geranylgeraniol can be used only in protein prenylation. The small GTP-binding protein RhoA, which may be important for cell spreading and cytokinesis, accumulated in the cytosol following treatment with lovastatin, suggestive of its inactivation. This effect was also prevented by the inclusion of either farnesol or geranylgeraniol in the incubation medium. Thus, lovastatin-induced apoptosis in mesangial cells occurs by interfering with prenylation dependent mitotic and post-mitotic events.
KW - Cytokinesis
KW - Farnesol
KW - Geranylgeraniol
KW - Mesangial cell
KW - Protein prenylation
KW - RhoA
UR - http://www.scopus.com/inward/record.url?scp=0030700682&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030700682&partnerID=8YFLogxK
U2 - 10.1016/S0167-4889(97)00091-8
DO - 10.1016/S0167-4889(97)00091-8
M3 - Article
C2 - 9398081
AN - SCOPUS:0030700682
VL - 1359
SP - 13
EP - 24
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 1
ER -