Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene

Tim H.M. Huang, James T. Quesenberry, Matthew B. Martin, Timothy S. Loy, Alberto A. Diaz-Arias

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed micro-satellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.

Original languageEnglish (US)
Pages (from-to)90-93
Number of pages4
JournalDiagnostic Molecular Pathology
Volume2
Issue number1
StatePublished - Jan 1 1993
Externally publishedYes

Keywords

  • Colorectal carcinoma
  • Formalin-fixed
  • Loss of heterozygosity
  • Microsatellites
  • Paraffin-embedded tissue

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene'. Together they form a unique fingerprint.

Cite this