Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene

Tim H.M. Huang; James T. Quesenberry; Matthew B. Martin; Timothy S. Loy; Alberto A. Diaz-Arias

Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene

Tim H.M. Huang, James T. Quesenberry, Matthew B. Martin, Timothy S. Loy, Alberto A. Diaz-Arias

Research output: Contribution to journal › Article › peer-review

Abstract

We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed micro-satellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.

Original language	English (US)
Pages (from-to)	90-93
Number of pages	4
Journal	Diagnostic Molecular Pathology
Volume	2
Issue number	1
State	Published - Jan 1 1993
Externally published	Yes

Keywords

Colorectal carcinoma
Formalin-fixed
Loss of heterozygosity
Microsatellites
Paraffin-embedded tissue

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

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Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene. / Huang, Tim H.M.; Quesenberry, James T.; Martin, Matthew B.; Loy, Timothy S.; Diaz-Arias, Alberto A.

In: Diagnostic Molecular Pathology, Vol. 2, No. 1, 01.01.1993, p. 90-93.

Research output: Contribution to journal › Article › peer-review

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AU - Martin, Matthew B.

AU - Loy, Timothy S.

AU - Diaz-Arias, Alberto A.

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N2 - We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed micro-satellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.

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KW - Formalin-fixed

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Loss of heterozygosity detected in formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using a microsatellite located within the deleted in colorectal carcinoma gene

Abstract

Keywords

ASJC Scopus subject areas

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