Abstract
We determined loss of heterozygosity from formalin-fixed, paraffin-embedded tissue of colorectal carcinoma using microsatellite polymorphism. The polymorphism was assayed based on DNA amplification by the polymerase chain reaction (PCR). The PCR-analyzed micro-satellite method was applied to assay degraded DNA extracted from paraffin-embedded blocks with adenocarcinoma of colon. The DNA from 26 tumors as well as their corresponding normal tissue samples were successfully amplified using a dinucleotide microsatellite located within an intron of the deleted in colorectal carcinoma gene. Allele losses on this marker were detected in 33% of informative colorectal carcinomas. This study demonstrates that microsatellites provide a powerful set of DNA markers for loss of heterozygosity on archival specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 90-93 |
| Number of pages | 4 |
| Journal | Diagnostic Molecular Pathology |
| Volume | 2 |
| Issue number | 1 |
| State | Published - Jan 1 1993 |
| Externally published | Yes |
Keywords
- Colorectal carcinoma
- Formalin-fixed
- Loss of heterozygosity
- Microsatellites
- Paraffin-embedded tissue
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Cell Biology