Longitudinal evaluation of polymorphonuclear leukocyte chemiluminescence in premature infants

Marcia S. Driscoll, Virginia L. Thomas, Rajam S. Ramamurthy, Daniel T. Casto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A chemiluminescence (CL) microassay was used to evaluate polymorphonuclear leukocyte (PMN) function in premature newborn infants longitudinally during a 2-month period and in healthy adult control subjects. At postnatal ages of 12, 26, 40 and 54 days the infants' mean peak CL activity was significantly lower than that of the adults. Infants with one or more low CL responses were more severely ill than those with normal CL activity. The infants with low CL responses had longer hospital stays and a higher frequency of serious infections, as well as more days of level 3 care, antimicrobial therapy, supplemental oxygen, assisted ventilation, and total parenteral nutrition. The PMN CL activity before, during, and after episodes of serious infection did not differ. In addition, a high frequency of depressed CL activity was observed at the time of infection. Our findings are consistent with previous studies suggesting that defective PMN oxidative metabolic responses are more common in neonates undergoing stress. Our results further suggest that defective PMN function may persist for the first 2 months of life and during the course of serious infection. Enhancement of PMN host defense may be an important strategy in the management of neonatal sepsis.

Original languageEnglish (US)
Pages (from-to)429-434
Number of pages6
JournalThe Journal of Pediatrics
Volume116
Issue number3
DOIs
StatePublished - 1990

Fingerprint

Luminescence
Premature Infants
Neutrophils
Infection
Newborn Infant
Total Parenteral Nutrition
Ventilation
Length of Stay
Oxygen

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Longitudinal evaluation of polymorphonuclear leukocyte chemiluminescence in premature infants. / Driscoll, Marcia S.; Thomas, Virginia L.; Ramamurthy, Rajam S.; Casto, Daniel T.

In: The Journal of Pediatrics, Vol. 116, No. 3, 1990, p. 429-434.

Research output: Contribution to journalArticle

Driscoll, Marcia S. ; Thomas, Virginia L. ; Ramamurthy, Rajam S. ; Casto, Daniel T. / Longitudinal evaluation of polymorphonuclear leukocyte chemiluminescence in premature infants. In: The Journal of Pediatrics. 1990 ; Vol. 116, No. 3. pp. 429-434.
@article{0c67894af0654a4988f7a91a6ef71797,
title = "Longitudinal evaluation of polymorphonuclear leukocyte chemiluminescence in premature infants",
abstract = "A chemiluminescence (CL) microassay was used to evaluate polymorphonuclear leukocyte (PMN) function in premature newborn infants longitudinally during a 2-month period and in healthy adult control subjects. At postnatal ages of 12, 26, 40 and 54 days the infants' mean peak CL activity was significantly lower than that of the adults. Infants with one or more low CL responses were more severely ill than those with normal CL activity. The infants with low CL responses had longer hospital stays and a higher frequency of serious infections, as well as more days of level 3 care, antimicrobial therapy, supplemental oxygen, assisted ventilation, and total parenteral nutrition. The PMN CL activity before, during, and after episodes of serious infection did not differ. In addition, a high frequency of depressed CL activity was observed at the time of infection. Our findings are consistent with previous studies suggesting that defective PMN oxidative metabolic responses are more common in neonates undergoing stress. Our results further suggest that defective PMN function may persist for the first 2 months of life and during the course of serious infection. Enhancement of PMN host defense may be an important strategy in the management of neonatal sepsis.",
author = "Driscoll, {Marcia S.} and Thomas, {Virginia L.} and Ramamurthy, {Rajam S.} and Casto, {Daniel T.}",
year = "1990",
doi = "10.1016/S0022-3476(05)82838-0",
language = "English (US)",
volume = "116",
pages = "429--434",
journal = "Journal of Pediatrics",
issn = "0022-3476",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - Longitudinal evaluation of polymorphonuclear leukocyte chemiluminescence in premature infants

AU - Driscoll, Marcia S.

AU - Thomas, Virginia L.

AU - Ramamurthy, Rajam S.

AU - Casto, Daniel T.

PY - 1990

Y1 - 1990

N2 - A chemiluminescence (CL) microassay was used to evaluate polymorphonuclear leukocyte (PMN) function in premature newborn infants longitudinally during a 2-month period and in healthy adult control subjects. At postnatal ages of 12, 26, 40 and 54 days the infants' mean peak CL activity was significantly lower than that of the adults. Infants with one or more low CL responses were more severely ill than those with normal CL activity. The infants with low CL responses had longer hospital stays and a higher frequency of serious infections, as well as more days of level 3 care, antimicrobial therapy, supplemental oxygen, assisted ventilation, and total parenteral nutrition. The PMN CL activity before, during, and after episodes of serious infection did not differ. In addition, a high frequency of depressed CL activity was observed at the time of infection. Our findings are consistent with previous studies suggesting that defective PMN oxidative metabolic responses are more common in neonates undergoing stress. Our results further suggest that defective PMN function may persist for the first 2 months of life and during the course of serious infection. Enhancement of PMN host defense may be an important strategy in the management of neonatal sepsis.

AB - A chemiluminescence (CL) microassay was used to evaluate polymorphonuclear leukocyte (PMN) function in premature newborn infants longitudinally during a 2-month period and in healthy adult control subjects. At postnatal ages of 12, 26, 40 and 54 days the infants' mean peak CL activity was significantly lower than that of the adults. Infants with one or more low CL responses were more severely ill than those with normal CL activity. The infants with low CL responses had longer hospital stays and a higher frequency of serious infections, as well as more days of level 3 care, antimicrobial therapy, supplemental oxygen, assisted ventilation, and total parenteral nutrition. The PMN CL activity before, during, and after episodes of serious infection did not differ. In addition, a high frequency of depressed CL activity was observed at the time of infection. Our findings are consistent with previous studies suggesting that defective PMN oxidative metabolic responses are more common in neonates undergoing stress. Our results further suggest that defective PMN function may persist for the first 2 months of life and during the course of serious infection. Enhancement of PMN host defense may be an important strategy in the management of neonatal sepsis.

UR - http://www.scopus.com/inward/record.url?scp=0025332333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025332333&partnerID=8YFLogxK

U2 - 10.1016/S0022-3476(05)82838-0

DO - 10.1016/S0022-3476(05)82838-0

M3 - Article

C2 - 2308036

AN - SCOPUS:0025332333

VL - 116

SP - 429

EP - 434

JO - Journal of Pediatrics

JF - Journal of Pediatrics

SN - 0022-3476

IS - 3

ER -