Long-lived species have improved proteostasis compared to phylogenetically-related shorter-lived species

Harrison Pride, Zhen Yu, Bharath Sunchu, Jillian Mochnick, Alexander Coles, Yiqiang Zhang, Rochelle Buffenstein, Peter J Hornsby, Steven N. Austad, Viviana I. Pérez

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Our previous studies have shown that the liver from Naked Mole Rats (NMRs), a long-lived rodent, has increased proteasome activity and lower levels of protein ubiquitination compared to mice. This suggests that protein quality control might play a role in assuring species longevity. To determine whether enhanced proteostasis is a common mechanism in the evolution of other long-lived species, here we evaluated the major players in protein quality control including autophagy, proteasome activity, and heat shock proteins (HSPs), using skin fibroblasts from three phylogenetically-distinct pairs of short- and long-lived mammals: rodents, marsupials, and bats. Our results indicate that in all cases, macroautophagy was significantly enhanced in the longer-lived species, both at basal level and after induction by serum starvation. Similarly, basal levels of most HSPs were elevated in all the longer-lived species. Proteasome activity was found to be increased in the long-lived rodent and marsupial but not in bats. These observations suggest that long-lived species may have superior mechanisms to ensure protein quality, and support the idea that protein homeostasis might play an important role in promoting longevity.

Original languageEnglish (US)
Pages (from-to)669-675
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume457
Issue number4
DOIs
StatePublished - Feb 20 2015

Fingerprint

Proteasome Endopeptidase Complex
Marsupialia
Rodentia
Autophagy
Heat-Shock Proteins
Proteins
Quality Control
Quality control
Mole Rats
Mammals
Ubiquitination
Fibroblasts
Starvation
Liver
Rats
Skin
Homeostasis
Serum

Keywords

  • Autophagy
  • Heat shock response
  • Long-lived species
  • Proteasome
  • Protein homeostasis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Long-lived species have improved proteostasis compared to phylogenetically-related shorter-lived species. / Pride, Harrison; Yu, Zhen; Sunchu, Bharath; Mochnick, Jillian; Coles, Alexander; Zhang, Yiqiang; Buffenstein, Rochelle; Hornsby, Peter J; Austad, Steven N.; Pérez, Viviana I.

In: Biochemical and Biophysical Research Communications, Vol. 457, No. 4, 20.02.2015, p. 669-675.

Research output: Contribution to journalArticle

Pride, H, Yu, Z, Sunchu, B, Mochnick, J, Coles, A, Zhang, Y, Buffenstein, R, Hornsby, PJ, Austad, SN & Pérez, VI 2015, 'Long-lived species have improved proteostasis compared to phylogenetically-related shorter-lived species', Biochemical and Biophysical Research Communications, vol. 457, no. 4, pp. 669-675. https://doi.org/10.1016/j.bbrc.2015.01.046
Pride, Harrison ; Yu, Zhen ; Sunchu, Bharath ; Mochnick, Jillian ; Coles, Alexander ; Zhang, Yiqiang ; Buffenstein, Rochelle ; Hornsby, Peter J ; Austad, Steven N. ; Pérez, Viviana I. / Long-lived species have improved proteostasis compared to phylogenetically-related shorter-lived species. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 457, No. 4. pp. 669-675.
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