Location of two cysteines in brain β1-tubulin that can be cross-linked after removal of exchangeable GTP

Melvyn Little, Richard F. Ludueña

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Two cysteines of the major neuronal β-tubulin subunit (β1) can be specifically cross-linked with the bifunctional sulfhydryl reagent N′,N′-ethylenebis(iodoacetamide) after removal of GTP. A cysteine in position 12 cross-links with one of the cysteines in position 201 or 211. Although the two cross-linked cysteines are separated by at least 189 residues in the primary structure of tubulin, they are maximally 9 Å apart in the tertiary structure.

Original languageEnglish (US)
Pages (from-to)28-33
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume912
Issue number1
DOIs
StatePublished - Mar 18 1987

Keywords

  • (Bovine brain)
  • Cysteine cross-link
  • Cysteine location
  • Tubulin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

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