Localization of the Nav1.8 sodium channel isoform at nodes of Ranvier in normal human radicular tooth pulp

Michael A. Henry, Howard J. Sorensen, Lonnie R. Johnson, S. Rock Levinson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The activation of voltage-gated sodium channels is necessary for action potential propagation and multiple sodium channel isoforms have been identified that show a differential distribution throughout the nervous system. An evaluation of sodium channel localization in the radicular pulp from normal human extracted third molars established the presence of the Nav1.8 isoform at nodes of Ranvier in a subpopulation of the myelinated axons as demonstrated with immunofluorescence confocal microscopy. A caspr antibody was used to identify the paranodal region of nodes of Ranvier and quantitative analysis revealed that 16.5% of the nodes contained significant Na v1.8 immunoreactivity. Since the Nav1.6 isoform has been described as the predominant sodium channel at essentially all nodes, the finding of Nav1.8 in a subpopulation of nodes suggests that multiple isoforms may coexist at some nodes of Ranvier and also suggests that this isoform may be an important nodal sodium channel type in the peripheral sensory nervous system of humans.

Original languageEnglish (US)
Pages (from-to)32-36
Number of pages5
JournalNeuroscience Letters
Volume380
Issue number1-2
DOIs
StatePublished - May 20 2005
Externally publishedYes

Fingerprint

Ranvier's Nodes
Sodium Channels
Tooth
Protein Isoforms
Voltage-Gated Sodium Channels
Third Molar
Peripheral Nervous System
Fluorescence Microscopy
Confocal Microscopy
Nervous System
Action Potentials
Axons
Antibodies

Keywords

  • Caspr
  • Human
  • Na1.8
  • Nodes of Ranvier
  • Sodium channels
  • Tooth pulp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of the Nav1.8 sodium channel isoform at nodes of Ranvier in normal human radicular tooth pulp. / Henry, Michael A.; Sorensen, Howard J.; Johnson, Lonnie R.; Levinson, S. Rock.

In: Neuroscience Letters, Vol. 380, No. 1-2, 20.05.2005, p. 32-36.

Research output: Contribution to journalArticle

Henry, Michael A. ; Sorensen, Howard J. ; Johnson, Lonnie R. ; Levinson, S. Rock. / Localization of the Nav1.8 sodium channel isoform at nodes of Ranvier in normal human radicular tooth pulp. In: Neuroscience Letters. 2005 ; Vol. 380, No. 1-2. pp. 32-36.
@article{0868bcc002f04cffa8792e32313e5e59,
title = "Localization of the Nav1.8 sodium channel isoform at nodes of Ranvier in normal human radicular tooth pulp",
abstract = "The activation of voltage-gated sodium channels is necessary for action potential propagation and multiple sodium channel isoforms have been identified that show a differential distribution throughout the nervous system. An evaluation of sodium channel localization in the radicular pulp from normal human extracted third molars established the presence of the Nav1.8 isoform at nodes of Ranvier in a subpopulation of the myelinated axons as demonstrated with immunofluorescence confocal microscopy. A caspr antibody was used to identify the paranodal region of nodes of Ranvier and quantitative analysis revealed that 16.5{\%} of the nodes contained significant Na v1.8 immunoreactivity. Since the Nav1.6 isoform has been described as the predominant sodium channel at essentially all nodes, the finding of Nav1.8 in a subpopulation of nodes suggests that multiple isoforms may coexist at some nodes of Ranvier and also suggests that this isoform may be an important nodal sodium channel type in the peripheral sensory nervous system of humans.",
keywords = "Caspr, Human, Na1.8, Nodes of Ranvier, Sodium channels, Tooth pulp",
author = "Henry, {Michael A.} and Sorensen, {Howard J.} and Johnson, {Lonnie R.} and Levinson, {S. Rock}",
year = "2005",
month = "5",
day = "20",
doi = "10.1016/j.neulet.2005.01.017",
language = "English (US)",
volume = "380",
pages = "32--36",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Localization of the Nav1.8 sodium channel isoform at nodes of Ranvier in normal human radicular tooth pulp

AU - Henry, Michael A.

AU - Sorensen, Howard J.

AU - Johnson, Lonnie R.

AU - Levinson, S. Rock

PY - 2005/5/20

Y1 - 2005/5/20

N2 - The activation of voltage-gated sodium channels is necessary for action potential propagation and multiple sodium channel isoforms have been identified that show a differential distribution throughout the nervous system. An evaluation of sodium channel localization in the radicular pulp from normal human extracted third molars established the presence of the Nav1.8 isoform at nodes of Ranvier in a subpopulation of the myelinated axons as demonstrated with immunofluorescence confocal microscopy. A caspr antibody was used to identify the paranodal region of nodes of Ranvier and quantitative analysis revealed that 16.5% of the nodes contained significant Na v1.8 immunoreactivity. Since the Nav1.6 isoform has been described as the predominant sodium channel at essentially all nodes, the finding of Nav1.8 in a subpopulation of nodes suggests that multiple isoforms may coexist at some nodes of Ranvier and also suggests that this isoform may be an important nodal sodium channel type in the peripheral sensory nervous system of humans.

AB - The activation of voltage-gated sodium channels is necessary for action potential propagation and multiple sodium channel isoforms have been identified that show a differential distribution throughout the nervous system. An evaluation of sodium channel localization in the radicular pulp from normal human extracted third molars established the presence of the Nav1.8 isoform at nodes of Ranvier in a subpopulation of the myelinated axons as demonstrated with immunofluorescence confocal microscopy. A caspr antibody was used to identify the paranodal region of nodes of Ranvier and quantitative analysis revealed that 16.5% of the nodes contained significant Na v1.8 immunoreactivity. Since the Nav1.6 isoform has been described as the predominant sodium channel at essentially all nodes, the finding of Nav1.8 in a subpopulation of nodes suggests that multiple isoforms may coexist at some nodes of Ranvier and also suggests that this isoform may be an important nodal sodium channel type in the peripheral sensory nervous system of humans.

KW - Caspr

KW - Human

KW - Na1.8

KW - Nodes of Ranvier

KW - Sodium channels

KW - Tooth pulp

UR - http://www.scopus.com/inward/record.url?scp=18044375589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18044375589&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2005.01.017

DO - 10.1016/j.neulet.2005.01.017

M3 - Article

C2 - 15854746

AN - SCOPUS:18044375589

VL - 380

SP - 32

EP - 36

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -