Localization of [125I]-insulin in subcellular particles of perfused rat liver

Walter F. Ward, Glenn E. Mortimore

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Rat livers were cyclically perfused for 30 min at 23°C with physiological concentrations of [125I]-insulin, the mitochondrial-lysosomal fraction was isolated and subjected to sucrose density gradient centrifugation. A peak of radioactive material, similar in size to native insulin, co-migrated with the peak of lysosomal marker enzyme on density gradients. The uptake of this material was blocked at low temperatures and Triton WR-1339 pretreatment failed to alter its distribution on the gradient. These findings indicate that the radioactive material in the peak represents [125I]-insulin, (or large fragments) that is associated with vesicles which compare with lysosomes in density but differ in Triton-storing capability.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume93
Issue number1
DOIs
Publication statusPublished - Mar 13 1980

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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