Abstract
Rat livers were cyclically perfused for 30 min at 23°C with physiological concentrations of [125I]-insulin, the mitochondrial-lysosomal fraction was isolated and subjected to sucrose density gradient centrifugation. A peak of radioactive material, similar in size to native insulin, co-migrated with the peak of lysosomal marker enzyme on density gradients. The uptake of this material was blocked at low temperatures and Triton WR-1339 pretreatment failed to alter its distribution on the gradient. These findings indicate that the radioactive material in the peak represents [125I]-insulin, (or large fragments) that is associated with vesicles which compare with lysosomes in density but differ in Triton-storing capability.
Original language | English (US) |
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Pages (from-to) | 66-73 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 93 |
Issue number | 1 |
DOIs | |
State | Published - Mar 13 1980 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology