Localization of [125I]-insulin in subcellular particles of perfused rat liver

Walter F. Ward; Glenn E. Mortimore

doi:10.1016/S0006-291X(80)80246-4

Localization of [¹²⁵I]-insulin in subcellular particles of perfused rat liver

Walter F. Ward, Glenn E. Mortimore

Barshop Institute

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Rat livers were cyclically perfused for 30 min at 23°C with physiological concentrations of [¹²⁵I]-insulin, the mitochondrial-lysosomal fraction was isolated and subjected to sucrose density gradient centrifugation. A peak of radioactive material, similar in size to native insulin, co-migrated with the peak of lysosomal marker enzyme on density gradients. The uptake of this material was blocked at low temperatures and Triton WR-1339 pretreatment failed to alter its distribution on the gradient. These findings indicate that the radioactive material in the peak represents [¹²⁵I]-insulin, (or large fragments) that is associated with vesicles which compare with lysosomes in density but differ in Triton-storing capability.

Original language	English (US)
Pages (from-to)	66-73
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	93
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(80)80246-4
State	Published - Mar 13 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Localization of [125I]-insulin in subcellular particles of perfused rat liver",

abstract = "Rat livers were cyclically perfused for 30 min at 23°C with physiological concentrations of [125I]-insulin, the mitochondrial-lysosomal fraction was isolated and subjected to sucrose density gradient centrifugation. A peak of radioactive material, similar in size to native insulin, co-migrated with the peak of lysosomal marker enzyme on density gradients. The uptake of this material was blocked at low temperatures and Triton WR-1339 pretreatment failed to alter its distribution on the gradient. These findings indicate that the radioactive material in the peak represents [125I]-insulin, (or large fragments) that is associated with vesicles which compare with lysosomes in density but differ in Triton-storing capability.",

author = "Ward, {Walter F.} and Mortimore, {Glenn E.}",

note = "Funding Information: The authors wish to express their gratitude to Dr. William W. Bromer of Eli Lily Laboratories for the gift of crystalline insulin. This work was supported by USPHS Grant AM25633 and was presented, in part, at the 38th Annual Meeting of The American Diabetes Association, Boston, MA., June, 1978.",

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AU - Ward, Walter F.

AU - Mortimore, Glenn E.

N1 - Funding Information: The authors wish to express their gratitude to Dr. William W. Bromer of Eli Lily Laboratories for the gift of crystalline insulin. This work was supported by USPHS Grant AM25633 and was presented, in part, at the 38th Annual Meeting of The American Diabetes Association, Boston, MA., June, 1978.

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AB - Rat livers were cyclically perfused for 30 min at 23°C with physiological concentrations of [125I]-insulin, the mitochondrial-lysosomal fraction was isolated and subjected to sucrose density gradient centrifugation. A peak of radioactive material, similar in size to native insulin, co-migrated with the peak of lysosomal marker enzyme on density gradients. The uptake of this material was blocked at low temperatures and Triton WR-1339 pretreatment failed to alter its distribution on the gradient. These findings indicate that the radioactive material in the peak represents [125I]-insulin, (or large fragments) that is associated with vesicles which compare with lysosomes in density but differ in Triton-storing capability.

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