TY - JOUR
T1 - Localization of septin proteins in the mouse cochlea
AU - Yoshida, Atsuhiro
AU - Yamamoto, Norio
AU - Kinoshita, Makoto
AU - Hiroi, Noboru
AU - Hiramoto, Takeshi
AU - Kang, Gina
AU - Trimble, William S.
AU - Tanigaki, Kenji
AU - Nakagawa, Takayuki
AU - Ito, Juichi
N1 - Funding Information:
This project was supported by a Grant-in-Aid for Young Scientists (B) (22791595) to NY, a Grant-in-Aid for Scientific Research (S) (23229009) to JI from the Ministry of Education, Culture, Sports, Science and Technology in Japan and Japan Society for Promotion of Science , and the National Institutes of Health (HD05311), National Alliance for Research on Schizophrenia and Depression Independent Investigator Award , and the Maltz Foundation to N.H.
PY - 2012/7
Y1 - 2012/7
N2 - Septins are a family of GTP binding proteins that are well conserved in eukaryotic species except plants. Septins contribute to the lateral compartmentalization of membranes, cortical rigidity, and the regulation of membrane trafficking by associating with membrane lipids, actin, and microtubules. The organ of Corti in the cochlea has pivotal roles in auditory perception and includes two kinds of highly polarized cells, hair and supporting cells, both of which are rich in actin and microtubules. To identify the roles of septins in the cochlea, we analyzed the localization of three septin proteins, septin 4 (SEPT4), septin 5 (SEPT5), and septin 7 (SEPT7) that are abundantly expressed in brain tissues, and also examined auditory functions of . Sept4 and . Sept5 null mice. SEPT4, SEPT5, and SEPT7 were expressed in inner and outer pillar cells and Deiters' cells but the distribution patterns of each protein in Deiters' cells were different. SEPT4 and SEPT7 were expressed in the phalangeal process where SEPT5 was not detected. In addition to these cells SEPT5 and SEPT7 were co-localized with presynaptic vesicles of efferent nerve terminals. Only SEPT7 was expressed in the cochlea at embryonic stages. Although expression patterns of septin proteins suggested their important roles in the function of the cochlea, both . Sept4 and . Sept5 null mice had similar auditory functions to their wild type littermates. Immunohistochemical analysis of . Sept4 null mice showed that compensatory expression of SEPT5 in the phalangeal process of Deiters' cells may have caused functional compensation of hearing ability in . Sept4 null mice.
AB - Septins are a family of GTP binding proteins that are well conserved in eukaryotic species except plants. Septins contribute to the lateral compartmentalization of membranes, cortical rigidity, and the regulation of membrane trafficking by associating with membrane lipids, actin, and microtubules. The organ of Corti in the cochlea has pivotal roles in auditory perception and includes two kinds of highly polarized cells, hair and supporting cells, both of which are rich in actin and microtubules. To identify the roles of septins in the cochlea, we analyzed the localization of three septin proteins, septin 4 (SEPT4), septin 5 (SEPT5), and septin 7 (SEPT7) that are abundantly expressed in brain tissues, and also examined auditory functions of . Sept4 and . Sept5 null mice. SEPT4, SEPT5, and SEPT7 were expressed in inner and outer pillar cells and Deiters' cells but the distribution patterns of each protein in Deiters' cells were different. SEPT4 and SEPT7 were expressed in the phalangeal process where SEPT5 was not detected. In addition to these cells SEPT5 and SEPT7 were co-localized with presynaptic vesicles of efferent nerve terminals. Only SEPT7 was expressed in the cochlea at embryonic stages. Although expression patterns of septin proteins suggested their important roles in the function of the cochlea, both . Sept4 and . Sept5 null mice had similar auditory functions to their wild type littermates. Immunohistochemical analysis of . Sept4 null mice showed that compensatory expression of SEPT5 in the phalangeal process of Deiters' cells may have caused functional compensation of hearing ability in . Sept4 null mice.
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U2 - 10.1016/j.heares.2012.04.015
DO - 10.1016/j.heares.2012.04.015
M3 - Article
C2 - 22575789
AN - SCOPUS:84861840415
SN - 0378-5955
VL - 289
SP - 40
EP - 51
JO - Hearing Research
JF - Hearing Research
IS - 1-2
ER -