Localization of proopiomelanocortin mRNA in functional subsets of neurons defined by their axonal projections

J. N. Wilcox, J. L. Roberts, B. M. Chronwall, J. F. Bishop, T. O'Donohue

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

In situ cDNA:mRNA hybridization is a technique that has been developed for the visualization of cDNA:mRNA hybrids in individual cells. To use this technique to answer questions of regulation in heterogeneous populations of cells in the brain, it must be combined with other procedures allowing for the identification of functional subgroups of neurons. We report here a procedure by which in situ cDNA:mRNA hybridization may be combined with retrograde axonal tracing using the fluorescent tracer fast blue. Using this technique, it now becomes possible to measure mRNA regulation in functional subsets of cells defined by their axonal projections.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalJournal of Neuroscience Research
Volume16
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • axonal projections
  • mRNA
  • neurons
  • proopiomelanocortin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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