Localisation of a series of intra-RNA cross-links in 16s RNA, induced by ultraviolet irradiation of escberichia coli 30s ribosomal subunits

Christian Zwieb, Richard Brimacombe

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-links can be isolated on a two-dimensional gel electrophoresis system, and subjected to sequence analysis. A series of these cross-linked complexes is described, and the cross-linked RNA regions are compared with the secondary structure derived for 16S RNA (see refs. 6, 7).

Original languageEnglish (US)
Pages (from-to)2397-2412
Number of pages16
JournalNucleic acids research
Volume8
Issue number11
DOIs
StatePublished - Jun 11 1980
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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