TY - JOUR
T1 - Localisation of a series of intra-RNA cross-links in 16s RNA, induced by ultraviolet irradiation of escberichia coli 30s ribosomal subunits
AU - Zwieb, Christian
AU - Brimacombe, Richard
N1 - Funding Information:
We are grateful to Dr. H. G. Wittmann for many helpful discussions, and to the Deutsche Forschungsgeraeinschaft for providing financial support.
PY - 1980/6/11
Y1 - 1980/6/11
N2 - Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-links can be isolated on a two-dimensional gel electrophoresis system, and subjected to sequence analysis. A series of these cross-linked complexes is described, and the cross-linked RNA regions are compared with the secondary structure derived for 16S RNA (see refs. 6, 7).
AB - Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-links can be isolated on a two-dimensional gel electrophoresis system, and subjected to sequence analysis. A series of these cross-linked complexes is described, and the cross-linked RNA regions are compared with the secondary structure derived for 16S RNA (see refs. 6, 7).
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U2 - 10.1093/nar/8.11.2397
DO - 10.1093/nar/8.11.2397
M3 - Article
C2 - 6160460
AN - SCOPUS:0019322293
VL - 8
SP - 2397
EP - 2412
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 11
ER -