Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury

M. Soledad Cepeda, Andrzej W. Lipkowski, Agnes Langlade, Patricia F. Osgood, H. Paul Ehrlich, Kenneth M Hargreaves, Stanislaw K. Szyfelbein, Daniel B. Carr

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To examine interactions between exogenous opioid analgesia and endogenous opioid generation at a site of burn-induced tissue injury, we measured β-endorphin (BE) and corticosterone (C) in aliquots of plasma and wound fluid withdrawn from subcutaneous wire mesh chambers beneath the site of a 3-5% surface area burn. After brief inhalational anesthesia at the time of thermal injury, rats received morphine (4 mg/kg, single dose), fentanyl (0.02 mg/kg hourly for 4 h), or no opioid. Systemic hormone responses and behavioral changes were minimal as expected for the minimal percentage burn. In all three groups intrachamber BE and C rose above baseline at 1, 2 and 4 h postburn, then returned to baseline at 24 h. Systemic opioid treatment produced analgesia (by tail flick latency testing) but did not reduce intrachamber hormone responses. Thus local BE and C responses at the site of thermal injury are regulated differently from systemic pituitary-adrenal responses.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalImmunopharmacology
Volume25
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Endorphins
Opioid Analgesics
Hot Temperature
Wounds and Injuries
Analgesia
Hormones
Fentanyl
Corticosterone
Burns
Morphine
Tail
Anesthesia

Keywords

  • Burn
  • Endorphin
  • Opioid
  • Stress response
  • Trauma

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cepeda, M. S., Lipkowski, A. W., Langlade, A., Osgood, P. F., Ehrlich, H. P., Hargreaves, K. M., ... Carr, D. B. (1993). Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury. Immunopharmacology, 25(3), 205-213. https://doi.org/10.1016/0162-3109(93)90049-V

Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury. / Cepeda, M. Soledad; Lipkowski, Andrzej W.; Langlade, Agnes; Osgood, Patricia F.; Ehrlich, H. Paul; Hargreaves, Kenneth M; Szyfelbein, Stanislaw K.; Carr, Daniel B.

In: Immunopharmacology, Vol. 25, No. 3, 1993, p. 205-213.

Research output: Contribution to journalArticle

Cepeda, MS, Lipkowski, AW, Langlade, A, Osgood, PF, Ehrlich, HP, Hargreaves, KM, Szyfelbein, SK & Carr, DB 1993, 'Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury', Immunopharmacology, vol. 25, no. 3, pp. 205-213. https://doi.org/10.1016/0162-3109(93)90049-V
Cepeda, M. Soledad ; Lipkowski, Andrzej W. ; Langlade, Agnes ; Osgood, Patricia F. ; Ehrlich, H. Paul ; Hargreaves, Kenneth M ; Szyfelbein, Stanislaw K. ; Carr, Daniel B. / Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury. In: Immunopharmacology. 1993 ; Vol. 25, No. 3. pp. 205-213.
@article{2d74ad4a442a4984ba20616ae0de4f49,
title = "Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury",
abstract = "To examine interactions between exogenous opioid analgesia and endogenous opioid generation at a site of burn-induced tissue injury, we measured β-endorphin (BE) and corticosterone (C) in aliquots of plasma and wound fluid withdrawn from subcutaneous wire mesh chambers beneath the site of a 3-5{\%} surface area burn. After brief inhalational anesthesia at the time of thermal injury, rats received morphine (4 mg/kg, single dose), fentanyl (0.02 mg/kg hourly for 4 h), or no opioid. Systemic hormone responses and behavioral changes were minimal as expected for the minimal percentage burn. In all three groups intrachamber BE and C rose above baseline at 1, 2 and 4 h postburn, then returned to baseline at 24 h. Systemic opioid treatment produced analgesia (by tail flick latency testing) but did not reduce intrachamber hormone responses. Thus local BE and C responses at the site of thermal injury are regulated differently from systemic pituitary-adrenal responses.",
keywords = "Burn, Endorphin, Opioid, Stress response, Trauma",
author = "Cepeda, {M. Soledad} and Lipkowski, {Andrzej W.} and Agnes Langlade and Osgood, {Patricia F.} and Ehrlich, {H. Paul} and Hargreaves, {Kenneth M} and Szyfelbein, {Stanislaw K.} and Carr, {Daniel B.}",
year = "1993",
doi = "10.1016/0162-3109(93)90049-V",
language = "English (US)",
volume = "25",
pages = "205--213",
journal = "Immunopharmacology",
issn = "0162-3109",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Local increases of subcutaneous β-endorphin immunoactivity at the site of thermal injury

AU - Cepeda, M. Soledad

AU - Lipkowski, Andrzej W.

AU - Langlade, Agnes

AU - Osgood, Patricia F.

AU - Ehrlich, H. Paul

AU - Hargreaves, Kenneth M

AU - Szyfelbein, Stanislaw K.

AU - Carr, Daniel B.

PY - 1993

Y1 - 1993

N2 - To examine interactions between exogenous opioid analgesia and endogenous opioid generation at a site of burn-induced tissue injury, we measured β-endorphin (BE) and corticosterone (C) in aliquots of plasma and wound fluid withdrawn from subcutaneous wire mesh chambers beneath the site of a 3-5% surface area burn. After brief inhalational anesthesia at the time of thermal injury, rats received morphine (4 mg/kg, single dose), fentanyl (0.02 mg/kg hourly for 4 h), or no opioid. Systemic hormone responses and behavioral changes were minimal as expected for the minimal percentage burn. In all three groups intrachamber BE and C rose above baseline at 1, 2 and 4 h postburn, then returned to baseline at 24 h. Systemic opioid treatment produced analgesia (by tail flick latency testing) but did not reduce intrachamber hormone responses. Thus local BE and C responses at the site of thermal injury are regulated differently from systemic pituitary-adrenal responses.

AB - To examine interactions between exogenous opioid analgesia and endogenous opioid generation at a site of burn-induced tissue injury, we measured β-endorphin (BE) and corticosterone (C) in aliquots of plasma and wound fluid withdrawn from subcutaneous wire mesh chambers beneath the site of a 3-5% surface area burn. After brief inhalational anesthesia at the time of thermal injury, rats received morphine (4 mg/kg, single dose), fentanyl (0.02 mg/kg hourly for 4 h), or no opioid. Systemic hormone responses and behavioral changes were minimal as expected for the minimal percentage burn. In all three groups intrachamber BE and C rose above baseline at 1, 2 and 4 h postburn, then returned to baseline at 24 h. Systemic opioid treatment produced analgesia (by tail flick latency testing) but did not reduce intrachamber hormone responses. Thus local BE and C responses at the site of thermal injury are regulated differently from systemic pituitary-adrenal responses.

KW - Burn

KW - Endorphin

KW - Opioid

KW - Stress response

KW - Trauma

UR - http://www.scopus.com/inward/record.url?scp=0027285123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027285123&partnerID=8YFLogxK

U2 - 10.1016/0162-3109(93)90049-V

DO - 10.1016/0162-3109(93)90049-V

M3 - Article

C2 - 8102620

AN - SCOPUS:0027285123

VL - 25

SP - 205

EP - 213

JO - Immunopharmacology

JF - Immunopharmacology

SN - 0162-3109

IS - 3

ER -