Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Harvey R. Schlesinger; Alan Frazer; Richard Friedman; Joe Mendels; Klaus Hummeler

doi:10.1016/0024-3205(79)90501-0

Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Harvey R. Schlesinger, Alan Frazer, Richard Friedman, Joe Mendels, Klaus Hummeler

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li⁺ ion rather than the ²²Na⁺ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li⁺ and ²²Na⁺ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li⁺ uptake. Thus, veratridine-dependent Li⁺ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na⁺ ion.

Original language	English (US)
Pages (from-to)	957-967
Number of pages	11
Journal	Life Sciences
Volume	25
Issue number	11
DOIs	https://doi.org/10.1016/0024-3205(79)90501-0
State	Published - Sep 11 1979
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1016/0024-3205(79)90501-0

Cite this

@article{798aa5aab3dc4790b801d5d9ca6e4946,

title = "Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells",

abstract = "Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.",

author = "Schlesinger, {Harvey R.} and Alan Frazer and Richard Friedman and Joe Mendels and Klaus Hummeler",

note = "Funding Information: This work was supported by National Institutes of Health grants CA-1345107 and CA-1448906, National Institute of Mental Health grants MH-25433 and MH-14654 and by research funds from the Veterans Administration .",

year = "1979",

month = sep,

day = "11",

doi = "10.1016/0024-3205(79)90501-0",

language = "English (US)",

volume = "25",

pages = "957--967",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "11",

}

TY - JOUR

T1 - Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

AU - Schlesinger, Harvey R.

AU - Frazer, Alan

AU - Friedman, Richard

AU - Mendels, Joe

AU - Hummeler, Klaus

N1 - Funding Information: This work was supported by National Institutes of Health grants CA-1345107 and CA-1448906, National Institute of Mental Health grants MH-25433 and MH-14654 and by research funds from the Veterans Administration .

PY - 1979/9/11

Y1 - 1979/9/11

N2 - Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.

AB - Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.

UR - http://www.scopus.com/inward/record.url?scp=0018718735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018718735&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(79)90501-0

DO - 10.1016/0024-3205(79)90501-0

M3 - Article

C2 - 513943

AN - SCOPUS:0018718735

SN - 0024-3205

VL - 25

SP - 957

EP - 967

JO - Life Sciences

JF - Life Sciences

IS - 11

ER -

Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this