Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Harvey R. Schlesinger; Alan Frazer; Richard Friedman; Joe Mendels; Klaus Hummeler

doi:10.1016/0024-3205(79)90501-0

Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Harvey R. Schlesinger, Alan Frazer, Richard Friedman, Joe Mendels, Klaus Hummeler

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li⁺ ion rather than the ²²Na⁺ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li⁺ and ²²Na⁺ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li⁺ uptake. Thus, veratridine-dependent Li⁺ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na⁺ ion.

Original language	English (US)
Pages (from-to)	957-967
Number of pages	11
Journal	Life Sciences
Volume	25
Issue number	11
DOIs	https://doi.org/10.1016/0024-3205(79)90501-0
State	Published - Sep 11 1979
Externally published	Yes

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(79)90501-0

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@article{798aa5aab3dc4790b801d5d9ca6e4946,

title = "Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells",

abstract = "Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.",

author = "Schlesinger, {Harvey R.} and Alan Frazer and Richard Friedman and Joe Mendels and Klaus Hummeler",

note = "Funding Information: This work was supported by National Institutes of Health grants CA-1345107 and CA-1448906, National Institute of Mental Health grants MH-25433 and MH-14654 and by research funds from the Veterans Administration .",

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doi = "10.1016/0024-3205(79)90501-0",

language = "English (US)",

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journal = "Life Sciences",

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TY - JOUR

T1 - Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

AU - Schlesinger, Harvey R.

AU - Frazer, Alan

AU - Friedman, Richard

AU - Mendels, Joe

AU - Hummeler, Klaus

N1 - Funding Information: This work was supported by National Institutes of Health grants CA-1345107 and CA-1448906, National Institute of Mental Health grants MH-25433 and MH-14654 and by research funds from the Veterans Administration .

PY - 1979/9/11

Y1 - 1979/9/11

N2 - Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.

AB - Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.

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ER -

Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Abstract

ASJC Scopus subject areas

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