Lithium ion uptake associated with the stimulation of action potential ionophores of cultured human neuroblastoma cells

Harvey R. Schlesinger, Alan Frazer, Richard Friedman, Joe Mendels, Klaus Hummeler

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Cultured human neuroblastoma cell lines were tested for the action potential sodium ionophore utilizing the Li+ ion rather than the 22Na+ ion. The cell lines studied included CHP-134, CHP-100, CHP-126, CHP-212 and LA-N-1. Veratridine-dependent uptake of Li+ and 22Na+ and its inhibition by tetrodotoxin implies the presence of the action-potential sodium ionophore. CHP-165, and undifferentiated tumor and RAJI a lymphoblast had no veratridine-dependent Li+ uptake. Thus, veratridine-dependent Li+ uptake provides a convenient means of assaying human neural cells for the action-potential sodium ionophore without the use of the radioactive Na+ ion.

Original languageEnglish (US)
Pages (from-to)957-967
Number of pages11
JournalLife Sciences
Volume25
Issue number11
DOIs
StatePublished - Sep 11 1979
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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