The authors have focused on the efficacy of liposome-encapsulated bovine hemoglobin (LEBH) in small animals. Circulation persistence in mice for LEBH is dose-dependent with half-lives measured at 15-20 hours for a normovolemic infusion of 25% blood volume. Modification of the LEBH surface with ganglioside GM1 results in a minimal increase in circulation persistence at this dose. Hemodynamic studies of chronically instrumented, conscious rats have shown that 10% normovolemic infusions can result in transient hematologic effects which are absent at 60 minutes post-infusion. Many of these effects have been eliminated by using synthetic distearoyl lecithin, and by coadministration of a PAF antagonist. Biodistribution data from 99mTc-LEBH in anesthetized rabbits show that 10% normovolemic loading results in 50% in the blood pool at 20 hours, 17% uptake in the liver, 15% in the spleen, 5% in the bladder, and trace levels in the kidney, brain, lung, and muscle. We have evaluated the storage stability and in vivo performance of freeze-dried LEBH, and initial results are favorable for the long-term storage of LEBH in the dry state. Manufacturing issues such as endotoxin levels, purity of LEBH components, and sterility have also been examined as possible variables in observed effects.
|Original language||English (US)|
|Number of pages||1|
|Journal||Biomaterials, Artificial Cells, and Immobilization Biotechnology|
|State||Published - Dec 1 1991|
|Event||8th World Congress of the International Society for Artificial Organs in conjunction with the 4th International Symposium on Blood Substitutes - Montreal, Que, Can|
Duration: Aug 19 1991 → Aug 23 1991
ASJC Scopus subject areas