Liposome encapsulated bovine hemoglobin; circulation persistence, biodistribution, hemodynamics and long-term storage

Alan S. Rudolph, Reuven Rabinovici, Giora Feurstein, Frances S. Ligler, Beth Goins, Richard Cliff, Robert Klipper, William Phillips

Research output: Contribution to journalConference article

Abstract

The authors have focused on the efficacy of liposome-encapsulated bovine hemoglobin (LEBH) in small animals. Circulation persistence in mice for LEBH is dose-dependent with half-lives measured at 15-20 hours for a normovolemic infusion of 25% blood volume. Modification of the LEBH surface with ganglioside GM1 results in a minimal increase in circulation persistence at this dose. Hemodynamic studies of chronically instrumented, conscious rats have shown that 10% normovolemic infusions can result in transient hematologic effects which are absent at 60 minutes post-infusion. Many of these effects have been eliminated by using synthetic distearoyl lecithin, and by coadministration of a PAF antagonist. Biodistribution data from 99mTc-LEBH in anesthetized rabbits show that 10% normovolemic loading results in 50% in the blood pool at 20 hours, 17% uptake in the liver, 15% in the spleen, 5% in the bladder, and trace levels in the kidney, brain, lung, and muscle. We have evaluated the storage stability and in vivo performance of freeze-dried LEBH, and initial results are favorable for the long-term storage of LEBH in the dry state. Manufacturing issues such as endotoxin levels, purity of LEBH components, and sterility have also been examined as possible variables in observed effects.

Original languageEnglish (US)
Number of pages1
JournalBiomaterials, Artificial Cells, and Immobilization Biotechnology
Volume19
Issue number2
StatePublished - Dec 1 1991
Externally publishedYes
Event8th World Congress of the International Society for Artificial Organs in conjunction with the 4th International Symposium on Blood Substitutes - Montreal, Que, Can
Duration: Aug 19 1991Aug 23 1991

ASJC Scopus subject areas

  • Medicine(all)

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