Lipidomics profiling of myelin

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Lipidomics is a powerful approach that can provide quantitative characterization of hundreds of lipid species from biological samples. Recent studies have highlighted the value of lipidomics to study myelin biology. This chapter provides a detailed description for the application of multidimensional mass spectrometry shotgun lipidomics (MDMS-SL) to neuroscience research and particularly to the analysis of brain lipidomes with a particular emphasis on myelin lipids, from sample preparation to bioinformatics analyses. Sample preparation includes brain sample harvesting, homogenization, and lipid extraction. Lipid content is determined and quantified, in an unbiased manner and with wide coverage, using MDMS-SL. Overall, the approach described herein is applicable for whole brain tissue or specific brain regions (e.g., hippocampus, cerebellum), and is expected to yield new insights on various aspects of myelin biology and lipid metabolism.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages37-50
Number of pages14
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1791
ISSN (Print)1064-3745

Keywords

  • Brain
  • Lipid metabolism
  • Mass spectrometry
  • Myelin
  • Shotgun lipidomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, C., Palavicini, J. P., & Han, X. (2018). Lipidomics profiling of myelin. In Methods in Molecular Biology (pp. 37-50). (Methods in Molecular Biology; Vol. 1791). Humana Press. https://doi.org/10.1007/978-1-4939-7862-5_4